User:Msmrugby/sandbox/Mike's Sandbox

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Week 5 Assignment

There is a new purification method for recombinant proteins that has been developed that is able to remove an affinity tag by inducing auto-cleavage. The new method utilizes the multiple domains of Ssp DnaB split Intein, and avoids the issue of premature cleaving of the proteins that have been tagged and expressed. According to a 2013 Shah article, "Split inteins are a class of naturally occurring proteins that carry out protein splicing in trans."