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Plan for Heat Shock Response Section

The heat-shock response in bacteria helps to stop any damage to the cellular processes in high temperature conditions. In response to high temperatures, heat-shock proteins, including chaperones and proteases are rapidly induced to protect against the denaturation of proteins within the bacteria. These chaperones help facilitate the folding of proteins within the cell to protect against rising temperatures. In most bacterial strains, sigma factor-32 ($$\sigma$$32) is responsible for regulating the heat-shock response. Sigma factor-32 is encoded by the rpoH gene and sits upstream of heat-shock genes.

ENVELOPE RESPONSE

The two-component signal transduction (2CST) system allows the bacterial cell to sense stress within the system. A histidine kinase that can be found in the cell's inner membrane detects the stress. The histidine kinase detects stress due to the autophosphorylation that initially occurs upon detection. Once the stress is detected, the system moves to a cytoplasmic response regulator. This is due to the cell being in a phosphate group, but this new response regulator will start to act like a transcription factor. This means that it will start to change what is expressed when looking at the genes. This is especially true when looking at the Cpx proteins which help to prevent the protein from folding the wrong way or not at all. Cpx proteins also help to ensure that there will be no other damage when looking at other cellular processes.

COLD SHOCK RESPONSE

When bacteria is in an area of very low and cold temperature, they will have a five hour long phase that will cause them not to grow at all. The way the bacteria tries to adapt is by creating child shock proteins that will be transcription factors that will be upregulated during the five hour phase. Once this five hour period ends, the bacteria will start to grow again, but it will be at a very slow rate. These proteins will help for the bacteria to continue to grow and survive at the lower temperatures. A protein called CspA was originally found in E. coli and is known to be one of the first cold shock proteins discovered and is known to be a single-stranded RNA. This will ultimately help with transcription and translation. With cold shock proteins, It is not just transcription and translation. You can also see that there can be condensation of the chromosome that occurs. This means that the cells will prematurely go onto the interphase stage of mitosis. There will also be an organization of the nucleoid which is when the intracellular and extracellular factors in the cell. And lastly, there is an enhancement when it comes to the survival of bacteria which helps the cell to get the food and nutrients it needs to survive.