User:Navneetktyagi/sandbox

Navneet K Tyagi, PhD (http://www.navneettyagi.com/) Navneet K Tyagi (born 1977) is an Indian American Scientist with expertise in the field of Protein folding, misfolding [1] [2] Neurodegenerative diseases [3], Rare and Ultra-rare Diseases [4], Membrane proteins [5] [6] Antibody development, Protein sciences, Molecular biology, Cell biology, Vaccine development, Protein/Antibody Engineering and upstream/downstream process development. Tyagi was born in Meerut, India on 13 July 1977. He completed his high school from St. Charles Inter College in 1994 and Bachelor in science from CCS University in 1997. He then completed his MS from University of Roorkee (Indian Institute of Technology) in 1999 and PhD (2005) at the Max Planck Institute of Molecular Physiology, Dortmund Germany. During his PhD study at Max Planck Institute of Molecular Physiology, Dortmund Germany under the supervision of Prof. Dr. Dr. Rolf Kinne he developed functional expression and purification system for the human Sodium/D-Glucose Co-transporter 1 (hSGLT1) [5], which is currently widely used in both the academic and industrial laboratories worldwide. In 2006 in he joined Howard Hughes Medical Institute/Yale Medical School as research associate with Prof. Dr. Arthur Horwich [7] [8], where he studies molecular mechanism of chaperonin (Hsp 60) GroEL/GroES [1], role of different molecular chaperones (Hsp110 and Hsc 70) on the Patho-mechanism of familial form of Amyotrophic Lateral Sclerosis (ALS, Lou Gehrig’s disease) [3]. At Yale Medical School he also discovered an ultra-rare neurodegenerative disease in a Turkish pedigree resulted due to recessive loss of function of the neuronal ubiquitin hydrolase UCHL1 [4]. Honors and Awards: •	Awarded Research Associate fellowship from Howard Hughes Medical Institute, Chevy Chase, MD, USA, 2006 – 2011 •	Recipient of Martin-Schmeisser fellowship from university of Dortmund, Germany, 2003 •	Awarded International Max Planck research school in chemical biology fellowship for PhD by Max Planck Institute for Molecular Physiology, Dortmund, Germany, 2002 – 2005 •	Awarded a Junior Research Fellowship from Department of Science (DST), New Delhi, India, 1999 – 2001

References: 1.	Navneet K Tyagi; Fenton, WA; and Horwich, AL (2009) GroEL/GroES cycling: ATP binds to an open ring before substrate protein favoring protein binding and production of the native state. PNAS 106, 20264-20269. 2.	Navneet K Tyagi; Fenton, WA; Deniz, AA; and Horwich, AL (2011) Double mutant MBP refolds at same rate in free solution as inside the GroEL/GroES chaperonin chamber when aggregation in free solution is prevented. FEBS Letter 585, 1969-1972. 3.	Song, Y; Nagy, M; Ni, W; Navneet K Tyagi; Fenton, WA; Lopez-Giraldez, F; Overton, J; Horwich, AL; and Brady, ST (2013). Molecular chaperone Hsp110 rescues a vesicle transport defect produced by an ALS-associated mutant SOD1 protein in squid axoplasm. PNAS 110, 5428-5433. 4.	Bilguvar, K; Navneet K Tyagi; Ozkara, C; Tuysuz, B; Bakircioglu, M; Caglayan, O; Baranoski, J; Erturk, O; Delil, S; Yalcinkaya, C; Karacorlu, M; Dincer, A; Johnson, MH; Mane, S; Chandra, S; Louvi, A; Boggon, T; Lifton, RP; Horwich, AL; and Gunel, M (2013). Recessive loss of function of the neuronal ubiquitin hydrolase UCHL1 leads to early-onset progressive neurodegeneration. PNAS 110, 3489-3494. 5.	Navneet K Tyagi; Kumar, A; Goyal, P; Pandey, D; Siess, W; and Kinne, RK (2007) D-Glucose-recognition and phlorizin-binding Sites in human sodium/D-glucose cotransporter1 (hSGLT1): A tryptophan scanning study. Biochemistry 46, 13616-13628. 6.	Navneet K Tyagi; Puntheeranurak, T; Raja, M; Kumar, A; Wimmer, B; Neundlinger, I; Gruber, H; Hinterdorfer; P; and Kinne, RK (2011) A biophysical glance at the outer surface of the membrane transporter SGLT1. Biochim. Biophys. Acta 1808, 1-18. 7.	Horwich, AL (2014) Molecular chaperones in cellular protein folding: the birth of a field. Cell 157(2):285-8. 8.	Horwich, AL (2011) Protein folding in the cell: an inside story. Nat Med. 17(10):1211-6.