User:Nihilisticdonut83/sandbox

The Michaelis-Menten constant is denoted by Km and is represented by the equation Km= (Koff + Kcat)/ Kon. The rates that the enzyme binds and dissociates from the substrate are represented by Kon and Koff respectively. Km is also defined as the substrate concentration at which enzymatic velocity reaches half of it's maximal rate. The tighter a ligand binds to a substrate, the lower the dissociation rate will be. It is important to know that Km and Koff are proportional, thus at higher levels of dissociation, the Michaelis-Menten constant will be larger.