User:Nkopplin/sandbox

What I did Paragraph
Over the course of the semester I edited a previously existing Wikipedia page covering Hydrogen Sulfide. My editing goal for this article was to make smaller additions as well as fill a few gaps left unanswered in the “Biosynthesis in the body” subsection which is part of a larger section titled “Production.”  This section refers to biosynthesis of hydrogen sulfide in broad terms, and fails to paint a complete picture of hydrogen sulfide synthesis by including two of the three known enzymes that are currently known to contribute to this biological process. What I particularly set out to fix was adding information about the important signaling role of hydrogen sulfide, so I felt that this provided a nice canvas to supplement and include relevant information I found. My sandbox reflects the final edits I made to the “Biosynthesis in the body” section, which I organized and placed directly below the current work posted on the hydrogen sulfide Wikipedia page which I titled “Biosynthesis in the body (currently in Wiki article)”. One of the important but smaller additions I made was my introduction sentence. In the end I kept the original introductory sentence for this section since I talked mostly about the enzymatic and non-enzymatic ways hydrogen sulfide is synthesized in biological systems. In the following sentence I briefly introduced the mechanism by which hydrogen sulfide alters biochemical activity within cells - which ties into the following paragraph that touches on the various physiological process’s hydrogen sulfide influences. Because hydrogen sulfide signaling is a relatively new field, scientist are still uncovering the mechanism behind hydrogen sulfide signaling in our body. I included a sentence that acted as a disclaimer, but I ended up ending half of the sentence out in my final peer revisions. Given this emerging field I was very cautious about the sources I used to support my work. I stuck closely to reviews and textbooks, keeping away from primary articles. I was cautioned early on by a Wikipedia reviewer to only include information that is widely known and accepted across the field, so his suggestion is reflected in the sources I chose. I have the required sources and cited in the appropriate manner which I referenced from the Wikipedia rubric. I am excited to see how the information I am planning on adding to the Wikipedia page helps to supplement and inspire enquiry about hydrogen sulfide signaling. Its nice to see that my work is finally up and people can see it! Hopefully it stays up fingers crossed!

1) Hydrogen Sulfide Chosen Article:
This is my topic: Hydrogen Sulfide

Choose at least 1 question relevant to the article you're evaluating and leave your evaluation on the article's Talk page. Be sure to sign your feedback with four tildes — ~.

What kinds of conversations, if any, are going on behind the scenes about how to represent this topic

 * a large range of topics: begins with health benefits, expands to various chemical properties of hydrogen sulfide that contributors wish to include, all the way to deleting a currents section talking about suicides by hydrogen sulfides (yikes).
 * The are several sections discussing external links modified.
 * none really discuss any biological roles hydrogen sulfide plays in biological systems.
 * looks like the most recent activity was July 11, 2014.

How is the article rated? Is it a part of any WikiProjects?

 * WikiProject Chemicals a daughter project of WikiProject Chemistry which aims to improve wikipedia's coverage of chemicals.
 * rated B-Class, Mid-Importance to this project.
 * WikiProject Geology, an attempt at creating a standardized informative comprehensive and easy-to-use geology resource.
 * rated C-Class, Low importance.

How does the way Wikipedia discusses this topic differ from the way we've talked about it in class?

 * Hydrogen sulfide is an endogenous gasotransmitter meaning: they're small molecules of endogenous gases that have physiological functions.
 * We have not yet covered gasotransmitters like nitric oxide or carbon monoxide, but we do know that small molecules can have a large effect of biological systems and are good for signal transduction.

Check a few citations. Do the links work? Does the source support the claims in the article?

 * Some of the citations are linked to websites, one of which is PennState Extension which is not a reputable article to cite with on Wikipedia.
 * Want: Sources known for fact-checking and neutrality, such as academic presses, peer-reviewed journals, or international newspapers.
 * Some links don't link to the academic articles they draw from.
 * Several sections: will have only one, or no citations with text: Biosynthesis in the body, Analytical Chemistry, Precursors to metal sulfides, Miscellaneous applications...

Is each fact referenced with an appropriate, reliable reference? Where does the information come from? Are these neutral sources? If biased, is that bias noted?

 * some websites (not reliable)
 * some are reports from news sources ex) Miami Herald (not reputable)
 * SDS sheets from various universities or sources (reliable)
 * mostly journals (reliable)
 * neutral tone

Is the article neutral? Are there any claims that appear heavily biased toward a particular position?

 * Overall a neutral tone is adopted by the article

Are there viewpoints that are overrepresented, or underrepresented?

 * Biological Significance of hydrogen sulfide is underrepresented.
 * incidents and suicide takes up a significant portion of the article.
 * talks extensively about harm, toxicity, safety.

Is everything in the article relevant to the article topic? Is there anything that distracted you?

 * No the suicide portion of the article seems to be insensitive. It goes into detail about suicide and how hydrogen sulfide was used as a concoction during a "suicide wave" in Japan. This should not be in the article.
 * They also talk extensively on the incidents or reported deaths by hydrogen sulfide. This does not seem to be appropriate to me to mention in the academic article.
 * the incidents are cited with reports from the Miami herald, Sun-Sentinel and ABC news which are not reputable sources to begin with.

Is any information out of date? Is anything missing that could be added?

 * More academic information could be added in general.
 * Biological role and functions of hydrogen sulfide.
 * endogenous gasotransmitter.
 * no image of pathway.
 * anti-inflammation
 * anti-cancer
 * anti-tumor
 * ion channel regulation
 * cardiovascular protection
 * antioxidant effects

Biosynthesis in the body (currently in Wiki article)
Hydrogen sulfide can be generated in cells via enzymatic or non enzymatic pathway. Hydrogen sulfide made by the non-enzymatic pathway is derived from proteins such as ferredoxins and Rieske proteins. Most hydrogen sulfide is produced from the enzymatic pathway. The endogenous hydrogen sulfide is produced by the enzymes cystathionine beta sy nthase (CBS) and cystathionine γ-lyase (CSE). L -Cysteine is the main substrate for hydrogen sulfide synthesis.

My work on: Biosynthesis in the body
Hydrogen sulfide can be generated in cells via enzymatic or non enzymatic pathway. acts as a gaseous signaling molecule which is known to inhibit Complex IV of the mitochondrial electron transport chain which effectively reduces ATP generation and biochemical activity within cells. Three enzymes are known to synthesize : cystathionine γ-lyase (CSE), cystathionine β-synthetase (CBS) and 3-mercaptopyruvate sulfurtransferase (3-MST). These enzymes have been identified in a breadth of biological cells and tissues, and their activity has been observed to be induced by a number of disease states. It is becoming increasingly clear that is an important mediator of a wide range of cell functions in health and in disease. CBS and CSE are the main proponents of biogenesis, which follows the trans-sulfuration pathway. These enzymes are characterized by the transfer of a sulfur atom from methionine to serine to form a cysteine molecule. 3-MST also contributes to hydrogen sulfide production by way of the cysteine catabolic pathway. Dietary amino acids, such as methionine and cysteine serve as the primary substrates for the transulfuration pathways and in the production of Hydrogen sulfide.

has been shown to be involved in physiological processes like vasoconstriction in animals, increasing seed germination and stress responses in plants. Hydrogen sulfide signaling is also innately intertwined with physiological processes that are known to be moderated by reactive oxygen species (ROS) and reactive nitrogen species (RNS). has been shown to interact with NO resulting in several different cellular effects, as well as the formation of a new signal called nitrosothiol. Hydrogen Sulfide is also known to increase the levels of glutathione which acts to reduce or disrupt ROS levels in cells. In these early days in the field of biochemistry and signaling there are more questions than answers.