User:Paulinad2022/Catalase

Assay Protocol
Experimental Protocol

=== The Catalase Assay is a form of enzymatic activity procedure that is a highly sensitive way of analyzing catalase activity. The total assay takes a total of about 40 minutes and is completed in a series of steps. In a given sample, the amount of catalase present can be detected by first placing the sample into the wells alongside control wells. The control wells as well as the sample of interest are then allowed to incubate at 25ºC for 5 min. Hydrogen peroxide solution is then added into the wells and further incubated for 30 more minutes. Stop solution is added in order to end the reaction, and a developer solution is mixed in and incubated for 10 minutes. A microplane reader is then utilized to analyze the results of the assay. ===