User:Pchokshi0612/sandbox

aton

Masatoshi Takeichi - Topic

Want to add in research about him discovering Cadherin and also where he worked

Discovery of Cadherin Family
While first working in Kyoto, Takeichi was using trypsin to study cell adhesions and aggregation. Once he moved to the Carnegie Institution, he realized the trypsin he was using behaved differently. This trypsin solution contained EDTA, along with the trypsin, which disturbed adhesion. The EDTA sequesters calcium ions, so Takeichi began testing for adhesion dependent on calcium. He used Chinese Hamster V79 cells and treated them with a variety of treatments, including EDTA, trypsin + EDTA, and trypsin + calcium. Once treated, these cells were tested for aggregation levels. He found that both calcium dependent and calcium independent pathways existed, and that the calcium treatment prevent trypsin's effects on the cell. (CITE)

Takeichi knew there was calcium dependent molecule that served an important role, but he had to find it. Rolf Kemler's anti serum was what helped him finally identify cadherin. E-cadherin was the first of the cadherin family to be discovered. During this research, Takeichi also observed changes in the morphology of the cells involved with adhesions. He accurately hypothesized that that the adhesions dependent on calcium played a role in managing cells' morphogenetic behavior. His discovery of the cadherin family led to a developing field of studying adhesions pathways. (CITE)