User:Peerzada Tajamul Mumtaz/sandbox

Peerzada Tajamul Mumtaz, born March 11, 1991 is a biochemist, known for his work on lncRNAs. Currently he is working as the Postdoctoral Fellow in Department of Nutrition and Health Sciences, University of Nebraska, USA. Tajamul grew up in Wasikhan, Rakhi Asham Sumbal Sonawari Bandipora, Jammu and Kashmir, India. He graduated from S.P. College Srinagar in 2011 and earned a Ph.D. from Jaipur National University, Jaipur Rajasthan in 2021. For his Ph. D., he joined Genomics lab at Faculty of Veterinary sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology (SKUAST) Kashmir, under the mentorship of Dr. Syed Mudasir Andrabi. During his tenure as a doctoral student, he learn to work effectively in the area of infectious diseases, cell culture, transcriptomics and lncRNA biology. During this tenure, he developed primary mammary epithelial cell culture from goat milk followed by their characterization using various methods like karyotyping, immunocytochemistry, PCR, and RT-PCR of various marker genes like Keratin 18 and CSN 2 (Patent published under application # 2019111013320 A) and commercialized the cells in the market. Followed by infection of these cells with bacteria he identified more than two thousand (2000) potential lncRNAs among which more than 1000 lncRNAs were novel. In addition, he became involved in projects that were focused to provide a comprehensive transcriptome and proteome profile of mammary gland epithelial cells at different stages of lactation and to find key differences in genes and pathways regulating milk traits between Jersey and Kashmiri cattle. And another project was aimed to investigate tissue-specific (liver, spleen and caecum) expression kinetics of NRAMP gene family (NRAMP1 and NRAMP2), interferon gamma (IFNγ), interleukin (IL-12, and IL-18) genes in chicken after Salmonella typhimurium challenge concomitant to clinical, blood biochemical and immunological parameters survey. During these projects, he became well versed with molecular biology techniques like RNA Isolation, cDNA synthesis, PCR, RT-PCR, Western blotting, Immunocytochemistry, Cell culture, Karyotyping and Bioinformatic analysis of transcriptomic and proteomic data. These projects have been fruitful and have resulted many scientific publications for him (https://scholar.google.co.in/citations?hl=en&user=n3qT4_0AAAAJ). In addition, his patent for “A Process of Isolating and Culturing of Primary Mammary Epithelial Cells (PMECs) From Caprine and Bovine Raw Milk” has been published.

Tajamul is an enthusiastic researcher, with more than five years of research experience in designing, executing, managing, presenting, writing and publishing research in biochemistry particularly cell biology, molecular biology and transcriptomics. Extensively trained in cell culture, cloning, gene expression and purification of proteins, data analysis and RNA biology. Proficient in multi-tasking, mentored interns and many graduate students. Successfully managed different projects to publications, monitored day to day workflow, staffing, documentation, data maintenance, project development, time-bound progress presentations at various platforms like weekly discussions, monthly group meetings and conferences. Efficiently managed and maintained the epithelial cell culture facility. Highly motivated and interested in genomics, molecular biology, lncRNA biology and biochemistry.