User:Pleusm/Three prime untranslated region proposal

Project Proposal
3’ UTR


 * The current Wikipedia page has a basic definition, mentions regulation with the polyA tail, and describes some regulatory sequences. These include a polyA signal, binding sites for proteins, and binding sites for micoRNAs. It has around 3 short paragraphs. This information will help us start, and we will expand upon it.
 * The talk page requested a clear, general definition at the beginning for someone who knows nothing about 3’ utr’s
 * history: when was it discovered? What did previous scientists think of its function?
 * physical: how the mechanism works (looping), how long the 3’ UTR is in different organisms, any patterns within it?
 * most common UTRs
 * types of regulation of 3’utr in different organisms
 * example: progenitors, 3’ UTRs inhibit the production of meiotic and oocyte proteins by post-transcriptional mechanisms involving two classes of RNA-binding proteins
 * microRNA regulation and polyA tail signal, relating to 3’ UTR and translational regulation– this is already mentioned in the wiki page
 * example study: human single nucleotide polymorphisms (SNPs) on both seed target region and the rest of 3′UTRs of miRNA target genes were under significantly stronger negative selection, when compared to non-miRNA target genes.
 * biology techniques used to study it in the past
 * effects of mutations
 * future research

Dividing up the work:


 * Bryce: biology techniques, future research, effects of mutations
 * Michael: history, physical characteristics, most common UTRs, general definition
 * Michelle: microRNA and poly (A) tail regulation of translation

Sources:


 * http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2585380/
 * The Influence of 3′UTRs on MicroRNA Function Inferred from Human SNP Data: http://www.hindawi.com/journals/cfg/2011/910769/
 * The power of the 3' UTR: translational control and development: http://www.ncbi.nlm.nih.gov/pubmed/12897774
 * mammalian gene regulation through the 3’UTR: http://dspace.mit.edu/handle/1721.1/42947
 * 3’ UTRs are the primary regulators of gene expression in the C. elegans germline: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2585380/
 * Characterization of 3'untranslated region (3'UTR) of the MICB gene: http://www.ncbi.nlm.nih.gov/pubmed/23380144
 * Data sheet: http://www.abc.hu/RNA/utr3.1.html
 * Translational control by the 3′-UTR: the ends specify the means: http://www.sciencedirect.com/science/article/pii/S0968000403000021