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The Institute of Nanoscopy is a research institute of Maastricht University established in 2014 that investigates cell structures at a macromolecular level. To understand the working mechanisms of protein complexes within cells, three-dimensional imaging of normal and disease-causing protein complexes is essential. The Institute also develops methods to capture the cell in close to native state and image whole cells and tissue slices down to 1 nanometer resolution. This way we can create maps of individual macromolecular complexes, by visualizing nanomachines in a close-to-physiological, cellular context. The institute’s key objective is to gain greater insight into the 3D form of cell proteins, thus paving the way for the development of more effective treatments for diseases such as cancer and tuberculosis. A better understanding of how protein complexes manage healthy, but also diseased, cells will allow drugs and vaccines to target problems more effectively. These developments will lead to scientific breakthroughs in the field of tuberculosis and cancer.