User:Po.indicwiki

This PO for Wiki from Hyderabad Sliding is done after fixing bacteria (smear) to the slide

(Heat kills and fixes the bacteria to the slide due to coagulation of bacterial protiens)

staining techniques

 * 1) simple staining
 * 2) negative staining
 * 3) differential staining
 * 4) impregnating methods

simple staining

simple staining provides color contrast but all the bacteria in snear are atained by same color

eg..,methylene blue,basic fuchsin

negative staining

here the background gets stained and bacteria are unstained

bacterial capusules doesnt take simple stains so by negative staining we can study them.

stain:Indian ink/nigrosin

differential stains

imparts different colors to different bacterial structures /bacteria

eg..,gram stain,acid fast stain