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Established methods for isolating Bacillus species for culture primarily involve suspension of sampled soil in distilled water, heat shock to kill off vegetative cells leaving primarily viable spores in the sample, and culturing on agar plates with further tests to confirm the identity of the cultured colonies. Additionally, colonies which exhibit characteristics typical of Bacillus bacteria can be selected from a culture of a significantly diluted and heat shocked or hot air dried environmental sample to potentially yield Bacillus bacteria for testing.