User:Rkpatel21/sandbox

History
Dr. Klaus-Armin's lab identified SHARP1 and SHARP2 as a novel subfamily in the BHLH protein family. They differentiated SHARP1 and SHARP2 from other BHLH-protein encoding genes since they are not transcribed until the end of embryonic development. The DNA sequence of SHARP1/Dec2 was first obtained by Dr. Yukia Kato's lab through a cDNA library search. Particularly, they obtained the sequence of Dec1 and conducted an EST search to identify the Dec2 sequence; the proteins shared 97% homology in the BHLH domain. After the identification of the Dec2 gene, Dr. Honma's lab identified its role as a regulator in the mammalian circadian clock.

structure
The BHLHE41 protein has a myc-type, basic helix-loop-helix (bHLH) domain and an orange domain. The orange domain is located on the carboxy-terminal end relative to the BHLH domain of the protein; the orange domain is a 30 residue sequence whose function is still unclear. . Members of the bHLH protein family are capable of dimerizing with each other to affect gene transcription through binding to specific DNA sequences. BHLHE41 protein also has alanine and glycine-rich regions in the C-terminal, and lacks the WRPW motif for interaction with the corepressor Groucho.