User:Rloviza/sandbox

Ion Torrent
Ion Torrent Systems Inc. developed a sequencing approach based on standard DNA replication chemistry. This technology measures the release of a hydrogen ion each time a base is incorporated. A microwell containing template DNA is flooded with a single nucleotide, if the nucleotide is complementary to the template strand it will be incorporated and a hydrogen ion will be released. This release triggers an ISFET ion sensor. If a homopolymer is present in the template sequence multiple nucleotides will be incorporated in a single flood cycle, and the detected electrical signal will be proportionally higher.

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454 Sequencing (in progress)
454 sequencing is a large-scale parallel pyrosequencing system. Single-stranded template DNA is fixed to streptavidin-coated beads throught the use of short adaptor sequences that have been ligated onto the ends of the sstDNA. Each bead is isolated and goes through Emulsion PCR which results in clonally amplified DNA fragments. __________________________________________________________________to be added to Genome(page) Sequencing (section)