User:Sadmanayub

Human Karyotype Lab


Introduction: The term karyotype refers to a photograph of the chromosomes of a cell at a time where each chromosomes still consists of two “sister” chromatids attached to each other at their centromeres. Purpose: To examine and prepare human karyotype chart

Hypothesis: Numbers or letters are having been assigned to specific chromosome pairs. Analysis of chromosome charts is helpful in study if chromosome abnormalities. Special staining techniques reveal light and dark regions (bands) on the chromosome. By matching the band, it is easy to identify specific chromosomes and prepare a chromosome chart.

Materials: 1) Sample Karyotype 2) Scissors 3) Unlined Paper 4) Transparent Tape 5) Glue

Procedure: Part I- The structure of a human karyotype chart. Part II- Chromosome Aberrations Part III- Prepare Karyotype Chart

Observation:

Part I- The structure of a human karyotype chart.

a) There are four rows of chromosomes that are in a karyotype chart.

b) An individual consist of 46 chromosomes.

c) 22 somatic chromosome pair and 1 sex chromosome pair

d) To name a karyotype, special staining technique reveal light and dark regions on the chromosome, also size, strands of line, are observed.

e) X, Y, they are the sex chromosomes.

f) For the homologous pair we get one from our mother and one from our father. A person who has X chromosomes is female, and a person with one X and one Y is considered male.

g) Photograph the chromosome of a cell at a time when each chromosome still consists of two sister chromatids attached to each other at their centromeres, prophase.

Part II- Chromosome Aberrations

h) An individual consist of 46 chromosomes. 22 somatic chromosome pair and 1 sex chromosome pair, which makes a total of 23 pairs.

i) Instead of the normal XX sex chromosomes for a female, only one X chromosome is present and fully functional. So there are 45 chromosomes in turner syndrome. Downs syndrome has a trisomy of #21, so when combined with a normal gamete from the other parent, the child ends up having 47 chromosomes.

j) The normal chromosome count comes up to 46 chromosomes, and these syndromes either lack or over chromosomes which creates effect on individuals. Part III- Prepare Karyotype Chart

k) Since the human on place has luck of no Y chromosomes and 1 X chromosomes, this person is considered a sterile female.

l) The total chromosome count is 45, and she has a case of Turner’s, which means she is missing an X chromosome, so this is not an expected number.

m) Since this person has a case of Turner’s syndrome, due to the lack of X chromosome, these are the following effect that might occur on this individual:

a. Sterile female

b. Undeveloped female characteristic

c. Short structure

d. Sometimes Below normal intelligence

Conclusion:

During this lab I learned to cooperate and also the concept of human karyotype. Now I am able to examine and prepare human karyotype chart of any given human karyotype. So basically, now this term makes a lot sense to me: The term karyotype refers to a photograph of the chromosomes of a cell at a time where each chromosomes still consists of two “sister” chromatids attached to each other at their centromeres.

P.S: THIS LAB WAS PERFORMED AND WRITTEN BY, SADMAN AYUB!

Sadmanayub 17:31, 22 August 2006 (UTC)Sadman Ayub