User:Shelby.slk600/Staphylococcus pseudintermedius

= Staphylococcus pseudintermedius = Staphylococcus pseudintermedius is a gram positive coccus bacteria of the genus Staphylococcus.

It is primarily a pathogen for domestic animals, seen commonly in dogs and cats, but has been known to affect humans as well. S. pseudintermedius is an opportunistic pathogen that secretes immune modulating virulence factors, has many adhesion factors, and the potential to create biofilms, all of which help to determine the pathogenicity of the bacterium. Diagnoses of Staphylococcus pseudintermedius have traditionally been made using cytology, plating, and biochemical tests. More recently, molecular technologies like MALDI-TOF, DNA hybridization and PCR have become preferred over biochemical tests for their more rapid and accurate identifications.

Identification
Staphylococcus intermedius group:

S. pseudintermedius, S. intermedius, S. delphini

Cytology
Using the gram stain technique, Staphylococci are easily identified by their clumped, gram positive, coccus morphology. Slides can be prepared directly using a patient swab, which lends to convenience in the clinic or classroom. However, given that S. pseudintermedius is prevalent within the normal microflora of numerous species, it is better identified as the agent of disease when a corresponding immune reaction is also observed. Where available, the need to identify immune reactors can be avoided by first inoculating the sample onto differential agar plates like SM110, which inhibit the growth of non-staphylococcus bacteria. Cytology alone does not allow for the differentiation between different species in the genus Staphylococci.

Plating
When plated on sheep or bovine blood agar, S. pseudintermedius displays incomplete ß-hemolysis. Colonies of S. pseudintermedius on sheep agar are described as medium in size and non-pigmented or grey-white. This can be useful for differentiating S. pseudintermedius from coagulase negative Staphylococci, and from S. aureus which tends to be yellow and display more variable hemolytic patterns on agar plates. S. pseudintermedius colonies are not hemolytic on equine blood agar. Plating is also limited in its ability to identify members of Staphylococci to the species level.

Biochemical Tests
Historically, biochemical tests have been an important tool used to discriminate between species of Staphylococci. Tests used to identify S. pseudintermedius specifically include DNase, hyaluronidase , coagulase, catalase, and acetoin production tests, amongst others. It can still be difficult to differentiate between members of the S. intermedius group using these methods alone; in veterinary medicine, such diagnoses have relied on the assumption that S. pseudintermedius is the known only member of this group to infect canine skin. More recently, studies using molecular identification methods have discovered that various strains of S. pseudintermedius have more phenotypic diversity than previously thought. It has been speculated that these differences have led to underestimation of the importance of S. pseudintermedius in human skin infections. Further, for this reason, S. pseudintermedius is no longer considered to be reliably identifiable using commercially available biochemical tests alone. More sensitive methods like MALDI-TOF have therefore since become preferred.

Identification of Methicillin-Resistance
Molecular methods, like MALDI-TOF and DNA hybridization, are the gold standard for accurately identifying the presence of mecA genes, which confer resistance to Beta-lactam drugs in S. pseudintermedius (a term coined "methicillin-resistance"). However, methicillin-resistance can still be identified reliably using biochemical or phenotypic methods, such as disc diffusion. Although cefoxitin disks have been used, oxacillin disks are considered to be much more sensitive, and thus a more accurate method for predicting methicillin resistance in S. pseudintermedius strains.

Epidemiology
In dogs, S. pseudintermedius is normally found on the microflora of the skin. The presence of S. pseudintermedius has been observed in higher amounts on dogs that suffer from atopic dermatitis. It is also one of the leading causes of bacterial skin and soft tissue infections, such as pyoderma, urinary tract infections , and surgical site infections. It is also known to infect cats, although not as common. It is transferred by animal-animal contact, and some dog-human zoonoses have also been reported. Transmission is done either vertically or horizontally. The overall prevalence of S. pseudintermedius in small animals is increasing every year, specifically in small animals worldwide.

S. pseudintermedius is becoming a threat due to its heterogeneous qualities and multi-drug resistance phenotype. Methicillin-resistant S. pseudintermedius (MRSP) has five major clonal complexe (CC) lineages, each with their own unique traits regarding genetic diversity, geographical distribution and antimicrobial resistance. The majority of all MRSP isolates were found in Europe and Asia, with North America, South America, and Oceania contributing only a small portion. The CC71 and CC258 lineages were mostly seen in Europe, CC68 was mostly seen in North America, and CC45 and CC112 seen in Asia. The top three antimicrobials worldwide that MRSP is found to be resistant to are erythromycin, clindamycin, and tetracycline.

When looking at the epidemiology of the Staphylococcus intermedius group (SIG), which includes S. pseudintermedius, S. intermedius, and S. delphini, it is noted that in humans most of the recorded cases were above the age of 50, diabetic, and/or immunocompromised in some way. Most of the cultures came from wound sites and respiratory specimens. S. pseudintermedius is not normally found within the microflora of humans. Humans that work in close proximity to animals are at higher risk of S. pseudintermedius infections, such as veterinarians, animal trainers, and zookeepers. Although the risk of pet owners becoming infected by their pets is low, there have been reported cases.

Pathogenicity and Virulence
As previously described, Staphylococcus pseudintermedius, an opportunistic pathogen, is a part of the normal microbiome of skin and mucous membranes in animals. Animals acquire this bacteria through vertical transmission. The strain of S. pseudintermedius colonizing the mother's vaginal mucous membrane is transferred during birth and becomes a part of the offspring's microbiome. A compromised immune system or tissue injury allows this bacteria to push past host defences and create an infection. We then seen clinical manifestations such as purulent dermatitis, otitis externa, conjunctivitis, urinary tract infections , and post-operative infections. Disease is most commonly seen in dogs and cats with canine pyoderma being the most notable manifestation of S. pseudintermedius.

The virulence of S. pseudintermedius is an area of on going research and has many unknowns. The virulence factors carried by S. pseudintermedius vary between strains and do not determine if the bacteria will cause an infection. Rather, infection is a result of an animal's immune status, environment, and genetics.

Numerous virulence factors such as enzymes, toxins, and binding proteins have been associated with S. pseudintermedius strains. These include proteases, thermonucleases, coagulases, DNAase, lipase, hemolysin, clumping factor, leukotoxin, enterotoxin, protein A, and exfoliative toxin.

Immune modulating virulence factors
Haemolysins, leukotoxins, exfoliative toxins, and enterotoxins are secreted from the bacteria to modulate the host's immune response.

The pore-forming cytotoxins, α-hemolysin and β-hemolysin, lyse erythrocytes of sheep and rabbits. Leukotoxin destroys host leukocytes and causes tissue necrosis. Exfoliative toxin is responsible for the majority of symptoms seen in canine pyoderma and otitis i.e. skin exfoliation and crusting. Exfoliative toxin causes vesicle formation and erosion in epithelial cells resulting in splitting of the skin. Super-antigens such as enterotoxins activate host immune cells causing T cell proliferation and cytokine release. This virulence factor induces vomiting and has been associated with food posioning in humans. Protein A, an immunoglobulin binding protein, has been found on the surface of S. pseudintermedius. Protein A attaches to the Fc region of host antibodies, rendering them useless. Without the Fc region, the host immune system cannot recognize that antibody; the complement system cannot be activated and phagocytes cannot destroy the bacteria.

Virulence factors for dissemination and adhesion
The previously mentioned protein A as well as clumping factor are surface proteins that allow the bacteria to bind to host cells. S. pseudintermedius has been found to produce biofilms, an extracellular matrix of protein, DNA, and polysaccharide, which aids the bacteria in avoiding the host immune system and resisting drugs. Biofilms allow the bacteria to persist on medical equipment even after disinfection and adhere to host cells, a component of chronic infections. Fragments of a biofilm can break off and disseminate to other sites in the body, spreading infection. Quorum sensing, a mechanism that coordinates the bacteria's colonization efforts, has been reported in some strains. Coagulase, lipase, and DNAase produced by the bacteria also aid in its dissemination throughout the host body. .

Zoonosis
Staphyloccus pseudintermedius has zoonotic potential as it has been found in humans that live with companion animals in the same household. S. pseudintermedius is not a normal commensal bacterium found in humans, however it is capable of adapting to the human microflora and has become increasingly more common. People whom are at the highest risk for contracting this pathogen are pet owners and veterinarians due to their higher contact with dogs and to a lesser extent cats. The most common place of colonization in the human body is within the nasal cavity and from here, the bacteria can cause infections. S. pseudintermedius infections in a human host have been known to cause endocarditis, post-surgical infections, inflammation of the nasal cavity (rhinosinusitis) and catheter-related bacteremia. Staphyloccus pseudintermedius becomes established in a human-wound, it has the ability to form antibiotic resistance biofilms. Mechanisms of biofilm resistance of S. pseudintermedius are likely multifactorial and may help to establish infections in humans.

Resistance in humans
There is an increasing prevalence of antibiotic resistance, specifically to methicillin of Staphyloccus pseudintermedius which makes it more challenging to treat when habituating a human host. Veterinary dermatologists are exposed to animals with skin and soft infections that commonly posses MRSP (methicillin‐resistant Staphylococcus pseudintermedius). Veterinarians have been found to be colonized with MRSP but not MSSP (methicillin‐susceptible S. pseudintermedius). Treatment of human MRSP infections is done with antibiotics and these should not be used for treatment in animals. Oral antimicrobial treatment for active infection is commonly done with the use of mupirocin, linezolid, quinupristin, rifampicin or vancomyocin are possible treatments. Hand washing, sterilizing equipment and hygiene practices should be implemented to decrease the spread of Staphylococcus infections.

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