User:Shrek4ver/sandbox

NlaIII is a restriction endonuclease.

Mechanism


NlaIII recognizes the palindromic and complementary DNA sequence of CATG/GTAC and it cuts outside of the G-C base pairs. This cutting pattern results in sticky ends with GTAC overhangs at the 3' end.

Uses
NlaIII is used in molecular biology techinques and experimental procedures such as:
 * Serial analysis of gene expression
 * Southern blotting
 * Molecular cloning
 * Restriction site mapping