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High-Information-Content Fingerprinting
High-Information-Content Fingerprinting (HICF) are a group of methods used for for the construction of physical maps of large genomes. These methods were developed from the acrylamide-based methods of Coulson et al. (1986) and Brenner and Livak (1989), with some adaptations to take advantage of automated sequencing technology, which lead to the increase of the throughput and sensitivity of fingerprinting.

All HICF methods share common features. HICF fragments are quite small, currently 500 bp or less due to the characteristics of DNA sequencing machines. Moreover, these methods use dye labeling to make the fragments detectable by the sequencers. The labels are dideoxy terminators (ddNTP) with a specific fluorescent dye for each base, end-labeled to recessed 3′-OH ends generated by either a type II or type IIS restriction enzyme.