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During the Parade of Nations at the 2023 Pan American Games opening ceremony, on October 20, 2023, athletes bearing the flags of their respective nations lead their national delegations as they paraded into National stadium in the host city of Santiago, Chile.

For the first time, each team had the option to allow two flag bearers, one male and one female. This was first done at the 2020 Summer Olympics, in an effort to promote gender equality.

March order
Athletes entered the stadium in an order dictated by tradition. As the host of the first Pan American Games, Argentina entered first. The Chilean delegation entered last, representing the host nation. The remaining countries entered in Spanish alphabetical order as per Pan American Sports Organization protocol.