User:Stone/sandbox

Abstract
A pyrolysis/sublimation technique was developed to isolate volatile amine compounds from a Mars soil analogue inoculated with V10 billion Escherichia coli cells. In this technique, the inoculated soil is heated to 500³C for several seconds at Martian ambient pressure and the sublimate, collected by a cold finger, then analyzed using high performance liquid chromatography.

Quotes

 * Martian soil that would yield 0.01 µg/g of ethylamine after pyrolysis

Important Points

 * LCMS as method.
 * Palagonite as sample
 * Escherichia coli
 * directly grown in water based medium on the substrate and cleaned centrifugation

Reference




Pyrolysis mass spectrometry (Py-MS) and in situ thermal hydrolysis-methylation (THM) of whole bacteria with tetramethylammonium hydroxide (TMAH) produced diagnostic fatty acid methyl ester (FAME) profiles for Pediococcus damnosus, P. dextrinicus, and Lactobacillus brevis.



Py-MAB-Tof detection and identification of microorganisms in urine metastable atom bombardment source (MAB)

pyrolysis time and temperature, gas atmosphere for pyrolysis Micrococcus luteus conversion of bacterial mass into chromatographic response was 0.28%.Bacillus mega-terium pyrolysed solution of bacteria



pyrolysis coupled to mass spec-trometry (Py-MS), for the detection and identification of bacteria in urine and other aqueous samplesis described. metastableatom bombardment source (MAB) Escherichia coli,Citrobacter freundii,Proteus mirabilisandPseudomonas aeruginosa,

Staphylococcus albus, Pseudomonas Juorescens and Bacillus subtilis



Bacillus anthracis (B0463), Bacillus cereus (B0037), Bacillus thuringiensis (B0158), Bacillus licheniformis (B0017), Bacillus subtilis (BOO14), Staphylo- coccus aureus, Staphylococcus albus, Pseudomonas jkorescens and Es- cherichia coli (type 0127)



Bacillusanthracis(BA) strain Texas andB. atrophaeus(formerlyB. subtilisvar.globigii)

Studies on the Whole Cell of Salmonella by Pyrolysis-Gas Chromatography-Mass Spectrometry