User:Swmcginn

Procedure

 * Open Image J software.
 * Open the desired image for analysis.
 * In Image open, select 8-bit
 * Select Image
 * Adjust
 * Threshold
 * Set the top slider to 175 and the bottom slider to 255.
 * Uncheck all other option.
 * Using the rectangle tool, select an area of the branch from the runner to the tip of the branch.
 * While editing the rectangle make note of the dimensions of it under the toolbar.
 * The total length and width of each branch will be identical in each picture.
 * Adjust the rectangle the length of the filled in section, ending the rectangle at where the branch first begins to stop filling.
 * Follow the Equation 1 below to find the fill percentage.


 * Fill % = $100* Length_{fill}⁄Length_{total}$			(Eq. 1)


 * Repeat steps for each branch.