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Characteristics
The genus Azospirillum belongs in the alpha-Proteobacteria class of bacteria in the family Rhodospirillaceae. Azospirillum are gram-negative, do not form spores, and have a slightly-twisted oblong-rod shape. Azospirillum have at least one flagellum and sometimes multiple flagella, which they use to move rapidly. Azospirillum are aerobic, but many can also function as microaerobic diazotrophs, meaning, under low oxygen conditions, they can change inert nitrogen from the air into biologically useable forms. At least three species, A. melinis, A. thiophilum, and A. humicireducens are facultative anaerobes, and can live, if necessary, without oxygen. Growth of Azospirillum is possible between 5°C and 42°C and in substrates with pH of 5 to 9, with optimal growth occurring around 30°C and a 7 pH. Microbiologists use nitrogen-free semi-solid media to isolate Azospirillum from samples. The most commonly used media is called "NFb".

Discovery and reclassification
The first species described in the genus was originally named Spirillum lipoferum in 1925 by M.W. Beijerinck. In Brazil during the 1970s, similar strains of this species were found associated with the roots of grain plants by scientists lead by Dr. Johanna Döbereiner. Her group discovered that these bacteria had the ability to fix nitrogen. Due to this discovery, Spirillum lipoferum was reclassified as Azospirillum lipoferum in 1978 by Jeffery Tarrand, Noel Krieg, and Döbereiner, who also added Azospirillum brasilense to the genus. By 2020, twenty-one species of Azospirillum had been described.

Origin of name
The prefix “Azo-” comes from the French word “azote”, which means nitrogen. This prefix is used to denote the ability of the bacteria to fix atmospheric nitrogen. The ending “-spirillum” refers to the shape of the bacteria which is similar to spiral-shaped bacteria in the genus Spirillum.

Ecological and agricultural significance
Azospirillum are found in freshwater and soil habitats, especially in close relationships with plant roots. (citation)  Associations with plants are thought to be largely beneficial. Over 113 species of plants in 35 different plant families have been documented to have benefited from association with a species of Azospirillum. In addition to vascular plants, the growth of the algae Chlorella vulgaris was positively affected by the presence of Azospirillum. Since the 1970s, Azospirillum strains have been researched for their effects in improving agricultural yields and improving growth of wild plants. In 2009, the first commercial inoculants containing Azospirillum came on the market, and by 2018, over 3 million doses were applied annually to crops by farmers, mainly in South America.

Plant growth promotion
Azospirillum promote plant growth through a variety of mechanisms. Many Azospirillum excrete plant hormones that alter how the roots of the plant grow. Affected roots frequently grow more branches and fine root hairs which may help the plants acquire water and nutrients more efficiently. In addition to these changes, Azospirillum can also alter the forms of nitrogen and phosphorus, two key plant nutrients, to make them more available to plants. However, how much nitrogen Azospirillum contribute to crop plants via biological fixation is debated. Azospirillum also make antioxidants that protect the plant roots from stresses due to drought and flooding.

Plant growth can also be promoted indirectly by Azospirillum reducing plant disease. Azospirillum competes with pathogens on the roots for space and resources such as iron. The plants' immune systems can also be primed by Azospirillum to resist attack by pathogens, a process known as induced systemic resistance.