User:Tim tien nu2002/Nucling

Nucling is an Apaf1-binding proapoptotic protein involved in apoptosome-mediated apoptosis. Luciferase assays have revealed that the activation of nuclear factor-κB induced by tumor necrosis factor-α, interleukin-1β and lipopolysaccharide is downregulated by the overexpression of Nucling in HEK293 cells. Moreover, the expression of endogenous cyclooxygenase 2, tumor necrosis factor-α and galectin-3, the end-point molecules in the pathway for the activation of nuclear factor-κB, as well as nuclear factor-κB (p65) itself, is upregulated in Nucling gene-deficient mouse embryonic fibroblasts, suggesting that nuclear factor-κB is a target of Nucling. Subsequent study has revealed that Nucling physically interacts with nuclear factor-κB (p65 and p50) and that the binding domain of Nucling is its amino-terminal region (amino acids 1-466) containing ankyrin repeats. Overexpression of Nucling prevents the translocation of nuclear factor-κB into the nucleus. In addition, the cytoplasmic retention of endogenous nuclear factor-κB in resting cells is not observed in Nucling gene-deficient mouse embryonic fibroblasts. These results reveal a novel function of Nucling as a suppressor of nuclear factor-κB, mediated by its cytoplasmic retention through physical interaction. http://www.jbc.org/cgi/content/abstract/279/39/41131