User:Tlizzie1/DNA–DNA hybridization

A method is introduced for hybridizing large numbers of DNA samples against large numbers of DNA probes on a single support membrane. Denatured DNA from up to 43 samples was fixed in separate lanes on a single membrane mounted in a Miniblotter 45. The membrane was then rotated 90 degrees in the same device, which enabled simultaneous hybridization with 43 different DNA probes. Hybridizations were also performed on lysates of bacterial cells blotted to membranes. A MiniSlot device allowed lysates loaded in parallel channels to be aspirated through the membrane, depositing horizontal lanes on the membrane surface. Hybridizations were performed in vertical lanes with either digoxigenin-labeled whole genomic probes or 16S rRNA-based oligonucleotide probes directly conjugated to alkaline phosphatase. The method permits the simultaneous determination of the presence of multiple bacterial species in single or multiple dental plaque samples, thus suggesting its usefulness for a range of clinical or environmental samples.

One method was introduced for hybridizing large numbers of DNA samples against large numbers of DNA probes on a single support membrane. The samples would have to be separated in their own lanes inside the membrane and then the membrane would have to be rotated to a different angle where it would result in simultaneous hybridization with many different DNA probes.

I will be drafting out a couple sentences to add to the Wikipedia article reviewing DNA hybridization