User:Tmmyn/Sequence assembly

Lead
In bioinformatics, sequence assembly refers to aligning and merging fragments from a longer DNA sequence in order to reconstruct the original sequence. This is needed as DNA sequencing technology is unable to read a whole genomes in one go, and rather reads small pieces of between 20 and 30,000 bases, depending on the technology used. Typically, the reads result from shotgun sequencing genomic DNA, or gene transcript (ESTs).

Sequence assembly presents a number of challenges, including dealing with repeated or duplicated fragments, technological errors, sample contamination.

Current sections:

1	Genome Assemblers

2	EST Assemblers

3	Types of Sequence Assembly

4	De-novo vs. Mapping Assembly

5	Sequence Assembly Pipeline (bioinformatics)

6	Influence of Technological Changes

7	Assembly Algorithms

8	Programs

9	See also

Proposed Sections:

1	History (address EST assemblers and influence of technological changes here)

2	Types of Sequence Assembly

3	Sequence Assembly Pipeline (bioinformatics)

4	Assembly Algorithms

5	Programs

6	See also