User:Tomásdearg92/sandbox

=Specific rotation: (Now Stale) Work in progress= In stereochemistry, the specific rotation [α] of a chemical compound is defined as the observed angle of optical rotation when plane-polarized light is passed through a sample with a path length of 1 dm and a sample concentration of 1 g/ml. It is the main property used to quantify the chirality of a molecular species or a mineral. The specific rotation of a pure material is an intrinsic property of that material at a given wavelength and temperature. The formal unit for specific rotation values is deg dm−1cm3 g−1 but values are reported in scientific literature as degrees. A positive value means dextrorotatory (clockwise) rotation and a negative value means levorotatory rotation.

Measurement
Optical rotation is measured with an instrument called a polarimeter. For a given wavelength there is a linear relationship between the observed rotation and the concentration of optically active compound in the sample. Values should be accompanied by the temperature at which the measurement was performed, assumed to be room temperature unless otherwise stated.


 * $$[\alpha]_\lambda^T = \frac{ \alpha}{l \times c}$$

In this equation, l is the path length in decimeters and c is the concentration in g/mL, for a sample at a temperature T (given in degrees Celsius) and wavelength λ (in nanometers).

For pure liquids, the density ρ (Greek letter "rho") in g/mL is equivalent to concentration, and the equation is expressed with ρ in place of c:


 * $$[\alpha]_\lambda^T = \frac{\alpha}{l \times \rho}$$

If the wavelength of the light used is 589 nm (the sodium D line), the symbol “D” is used for the wavelength, as in the expression [α]D. The rotation is reported using degrees, and the sign of the rotation (+ or −) is always given.

When using this equation, the concentration and the solvent may be provided in parentheses after the rotation. No units of concentration are given (it is assumed to be g/100mL).

If a 1% w/v solution of a rotating substance in ethanol gave a clockwise rotation of 6.2° dm-1 cm3 g-1 when measured at 20 °C with light from a sodium lamp, this would be expressed as follows:


 * $$[\alpha]_D^{20} = +6.2$$° (c 1.0, EtOH)

Dealing with large and small rotations
If the specific rotation is very or the sample is very concentrated, the actual rotation of the sample may be greater than 180°. A single polarimeter measurement cannot detect when this has happened (for example, the values +270° and −90° are indistinguishable, nor are the values 361° and 1°). In these cases, varying the concentration or path length allows one to determine the true value.

In cases of very small or very large angles, one can also use the variation of specific rotation with wavelength to facilitate measurement. Switching wavelength is particularly useful when the angle is small. Many polarimeters are equipped with a mercury lamp (in addition to the sodium lamp) for this purpose.

Mixtures
In theory, the optical purity of a sample containing a mixture of enantiomer s can be determined from the measured optical rotation. For example, if a sample of 2-bromobutane measured under standard conditions has an observed rotation of −9.2°, this indicates that the net effect is due to 9.2°/23.1°=40% of the R enantiomer. This value (40%) is called the enantiomeric excess. The remainder of the sample is a racemic mixture of the enantiomers (30% R and 30% S), which has no net contribution to the observed rotation. The total concentration of the R enantiomer is 70%. The utility of this method is limited as the presence of small amounts of highly rotating impurities can greatly affect the rotation of a given sample. Moreover, the optical rotation of a compound may not be linearly dependent on its enantiomeric excess because of aggregation in solution. Other methods of determining the enantiomeric ratio such as gas chromatography or HPLC with a chiral column are generally preferred.

Absolute configuration
The variation of specific rotation with wavelength is the basis of optical rotatory dispersion (ORD), an analytical technique that can be used to elucidate the absolute configuration of certain compounds.

Solutions and mixtures

 * $$[\alpha]_\lambda^T = \frac{ \alpha}{l \times c}$$

In this equation, α (Greek letter "alpha") is the measured rotation in degrees, l is the path length in decimeters, c is the concentration in g/mL, T is the temperature at which the measurement was taken (in degrees Celsius), and λ is the wavelength in nanometers.

For practical and historical reasons, concentrations are often reported in units of g/100mL. In this case, a correction factor in the numerator is necessary:


 * $$[\alpha]_\lambda^T = \frac{ 100 \times \alpha}{l \times c}$$

When using this equation, the concentration and the solvent may be provided in parentheses after the rotation. The rotation is reported using degrees, and no units of concentration are given (it is assumed to be g/100mL). The sign of the rotation (+ or −) is always given. If the wavelength of the light used is 589 nanometer (the sodium D line), the symbol “D” is used. If the temperature is omitted, it is assumed to be at standard room temperature (20 °C).

For example, the specific rotation of a compound would be reported in the scientific literature as:


 * $$[\alpha]_D^{20} +6.2$$° (c 1.00, EtOH)

Pure liquids
For a pure liquid, the mass concentration of that liquid is equivalent to the mass density. Therefore d or ρ (Greek letter "rho"), the density of the liquid in g/mL, is presented in this form of the equation instead of c.


 * $$[\alpha]_\lambda^T = \frac{\alpha}{l \times \rho}$$

The term neat may be used to indicate a pure liquid, and the density of the liquid reported. For example, the specific rotation of trans-(−)-(2S,3S)-2,3-dimethyloxirane would be reported as follows:


 * $$[\alpha]_D^{25} -58.8$$° (neat), $$d_4^{25} = 0.7998$$

Solids
As specific rotation experiments are often performed in quartz cells, knowing the specific rotation of quartz itself is important to obtain accurate measurements for the sample in question. (This is true, but only amounts to WP:Synthesis unless a WP:RS is given.)

Normal quartz plate
In the field of saccharimetry, a normal quartz plate has replaced the normal sugar solution as a reference standard. The normal sugar solution contains 26 g sucrose in 100 ml water and has a specific rotation ([α]D20) of 66.529°, or 100 °S on the International Sugar Scale. The thickness of the normal quartz plate is between 1.5934−1.5940 mm.

Thin films
The specific rotation of a thin film may be measured by treating the film as a thin plate. The film is first cast on an optically transparent and non-rotating material such as glass, and the thickness (path length) of the film measured using a tool such as a micrometer.

Powders and suspensions
Suspensions of crystalline solids and polymers are stirred constantly during measurement in a specially designed quartz cell. Powders are sieved to a suitable grain size, and suspended in a liquid with a refractive index equal to that of the powder. The differences in specific rotation between dissolved and suspended samples may demonstrate additional rotation due to the assembly of molecules in the solid phase, or due to the secondary structure of molecules such as RNA.

Gases
The specific rotations of gaseous samples are used to illustrate and quantify solvent effects by comparison to the measurement obtained in solution. Note to self: The reported + and - pinene do not appear to be enantiomers (?)

References to be incorporated:
Some of them refer to the specific rotation explicitly, some refer simply to "optical rotation" but then present [α]D or [α]T values.
 * Vogl. The specific rotation of polymers is measured in solution, suspension and as thin films. Suspensions are stirred during measurements in a quartz cell, the suspending medium is chosen to match the refractive index of the suspended solid. Solutions measured with a path length of 10 cm. Thickness of polymer films measured using a micrometer.
 * The phrase neat is used in place of the concentration remark for pure liquids. $$[\alpha]_\lambda^T = \frac{\alpha}{l \times d}$$ for neat liquids and solids. Solutions are described as in the article, the correction factor, for practical reasons. "Rather than stating the correct CGS units it is now agreed that any reference to a dimension is avoided." Includes recommendations for optical purity calculations and information on ORD. Gases mentioned.
 * Crystalline quartz. Secondary source on specific rotation at various wavelengths (ORD). $$[\alpha] = \frac{10^4 \times \alpha}{d \times C}$$ d in mm, C in g/ 100 cm3. Rest of info is on Faraday effect.
 * Very detailed and extremely long. Focuses on saccharimetry. Describes the normal sugar solution, and the normal quartz plate. Also reccommends using the mercury line instead of the sodium D line for measurements, for various reasons.
 * Vogl. Includes diagram of apparatus for the measurement of the specific rotations of suspensions. Describes the sieving procedure. Differences between solid state and dissolved RNA, for example.
 * personal essay? Otto Vogl is quite prolific in this area, or so it seems. A review by him could be ideal.
 * Here we go...
 * The International Pharmacopoeia tends to muddy the waters with the introduction of several variables that appear to cancel down to concentration, albeit with correction factors and unit changes thrown in to the bargain. "[C]alculated with reference to a layer 100 mm [1 dm] thick, and divided by the relative density (specific gravity)..." $$[\alpha]^t_\lambda = \frac{10000 a}{l c} = \frac{10000 a}{l d p}$$ l in mm, c in g/ 100ml, d is relative density, p in g/ 100g (That makes dp g/100ml). Also mentions molar rotation and SI units.
 * Dispenses with much of the bittiness of the Pharmacopoeia, while also swapping wavelength and temperature, i.e. $$[\alpha]^\lambda_t$$ . Detailed recommended procedure and error reduction included.
 * And then, a phycics book comes along and dispenses with the standards used in chemistry and pharmacy (and it is likely other physics books do the same). Introduces in a form similar to the Beer-Lambert-Bouger Law, and explains that additivity may apply, but secondary effects cause issues with mixtures. $$\theta = \alpha_\lambda C l$$ for solutions, $$\theta = \alpha_\lambda l$$ for solids and "homogeneous liquids".
 * Gas phase measurements, and comparison with solution- used to demonstrate solvent effects on specific rotation. Temperature dependance of various terpene derivatives.

Dealing with large and small rotations
If a compound has a very large specific rotation or a sample is very concentrated, the actual rotation of the sample may be larger than 180°, and so a single polarimeter measurement cannot detect when this has happened (for example, the values +270° and −90° are not distinguishable, nor are the values 361° and 1°). In these cases, measuring the rotation at several different concentrations allows one to determine the true value. Another method would be to use shorter path-lengths to perform the measurements.

In cases of very small or very large angles, one can also use the variation of specific rotation with wavelength to facilitate measurement. Switching wavelength is particularly useful when the angle is small. Many polarimeters are equipped with a mercury lamp (in addition to the sodium lamp) for this purpose.

=Specific rotation: Current article= Specific rotation

=Other Stuff=

TAFI: Ideas
For the Wikiprojects (in this case Food and Drink):

And on the nominations page, slightly extended to:

Primitive Irish – (page view stats • edit • talk • history) – WikiProject Languages • Ireland • Middle Ages • Celts
 * Nom. --Nominator signature
 * 1) Support --Supporter signature