User:Unbipentium/Deoxyadenosine triphosphate

Enzymatic synthesis of deoxyadenosine triphosphate
Deoxyadenosine triphosphate is able to be enzymatically synthesized with DNA as the starting material using deoxyribonuclease (DNase), nuclease P1, adenylate kinase, and pyruvate kinase. The synthesis starts with the heat denaturation of DNA followed by treatment with DNase I to produce oligomers. Next, the solution is treated with nuclease P1 to form deoxynucleoside monophosphates. Using a mixture of adenylate kinase and pyruvate kinase, the deoxyadenosine monophosphate was selectively converted to dATP. After purification, a purity of 90%-95% can be achieved using this method of synthesis with a 40% overall yield.

In immunocompromised individuals
Deficiency of the enzyme adenosine deaminase is known to cause immunodeficiency in individuals. Research has found that dATP may be a potential toxic metabolite in adenosine deaminase deficiency. Patients in the study who were immunodeficient and adenosine deaminase deficient were found to have over 50 times the levels of dATP in their erythrocytes compared to non-immunodeficient, adenosine deaminase deficient patients. This is abnormal and provides evidence that increased erythrocyte dATP levels are the toxic metabolites responsible for immune system deficiency in individuals with adenosine deaminase deficiency. Infusion of normal, non-enzyme deficient erythrocytes resulted in the loss of dATP in the erythrocytes of these individuals.