User:Vengeanceknight/sandbox

Original- "Geobacter"

History
Geobacter metallireducens was first isolated by Derek Lovley in 1987 in sand sediment from the Potomac River in Washington D.C. The first strain was deemed strain GS-15.

Applications
Geobacter's ability to consume oil-based pollutants and radioactive material with carbon dioxide as waste byproduct has been used in environmental clean-up for underground petroleum spills and for the precipitation of uranium out of groundwater. Geobacter metabolize the material by creating electrically conductive pili between itself and the food material.

Multiple Geobacter species cooperate in metabolizing a mixture of chemicals that neither could process alone. Provided with ethanol and sodium fumarate, G. metallireducens broke down the ethanol, generating an excess of electrons that were passed to G. sulfurreducens via "nanowires" grown between them, enabling G. sulfurreducens to break down the fumarate ions. The nanowires are made of proteins with metal-like conductivity.

Microgravity
Geobacter has been tested in microgravity and this does not make a substantial difference.

Biodegradation and bioremediation
Microbial biodegradation of recalcitrant organic pollutants is of great environmental significance and involves intriguing novel biochemical reactions. In particular, hydrocarbons and halogenated compounds have long been doubted to be anaerobically degradable, but the isolation of hitherto unknown anaerobic hydrocarbon-degrading and reductively dehalogenating bacteria documented these processes in nature. Novel biochemical reactions were discovered, enabling the respective metabolic pathways, but progress in the molecular understanding of these bacteria was slowed by the absence of genetic systems for most of them. However, several complete genome sequences later became available for such bacteria. The genome of the hydrocarbon degrading and iron-reducing species G. metallireducens (accession nr. NC_007517) was determined in 2008. The genome revealed the presence of genes for reductive dehalogenases, suggesting a wide dehalogenating spectrum. Moreover, genome sequences provided insights into the evolution of reductive dehalogenation and differing strategies for niche adaptation.

Edited- "Geobacter"

History (Unchanged)
Geobacter metallireducens was first isolated by Derek Lovley in 1987 in sand sediment from the Potomac River in Washington D.C. The first strain was deemed strain GS-15.

Metabolic Mechanisms (Newly Added)
For quite some time, it was thought that Geobacter species lacked c-cytochromes that can be utilized to reduce metal ions, hence it was assumed that they required direct physical contact in order to use metal ions as terminal electron acceptors (TEAs). The discovery of the highly conductive pili in Geobacter species, and the proposal of using them as biological nano-wires further strengthened this view. Nevertheless, recent discoveries have revealed that many Geobacter species, such as G. uraniireducens, not only do not possess highly conductive pili, but also do not need direct physical contact in order to utilize the metal ions as TEAs, suggesting that there is a great variety of extracellular electron transport mechanisms among the Geobacter species. For example, one other way of transporting electrons is via a quinone-mediated electron shuttle.

Another interesting metabolic phenomenon is the cooperation between Geobacter species, in which several species cooperate in metabolizing a mixture of chemicals that neither could process alone. Provided with ethanol and sodium fumarate, G. metallireducens broke down the ethanol, generating an excess of electrons that were passed to G. sulfurreducens via "nanowires" grown between them, enabling G. sulfurreducens to break down the fumarate ions. The nanowires are made of proteins with metal-like conductivity.

Applications (Edited)
Geobacter's ability to consume oil-based pollutants and radioactive material with carbon dioxide as waste byproduct has been used in environmental clean-up for underground petroleum spills and for the precipitation of uranium out of groundwater. Geobacter metabolize the material by creating electrically conductive pili between itself and the food material.

Biodegradation and bioremediation
Microbial biodegradation of recalcitrant organic pollutants is of great environmental significance and involves intriguing novel biochemical reactions. In particular, hydrocarbons and halogenated compounds have long been doubted to be anaerobically degradable, but the isolation of hitherto unknown anaerobic hydrocarbon-degrading and reductively dehalogenating bacteria documented these processes in nature. Novel biochemical reactions were discovered, enabling the respective metabolic pathways, but progress in the molecular understanding of these bacteria was slowed by the absence of genetic systems for most of them. However, several complete genome sequences later became available for such bacteria. The genome of the hydrocarbon degrading and iron-reducing species G. metallireducens (accession nr. NC_007517) was determined in 2008. The genome revealed the presence of genes for reductive dehalogenases, suggesting a wide dehalogenating spectrum. Moreover, genome sequences provided insights into the evolution of reductive dehalogenation and differing strategies for niche adaptation.

Vengeanceknight (talk) 01:17, 7 October 2017 (UTC)

Final Edit of- "Geobacter"

History (Unchanged)
Geobacter metallireducens was first isolated by Derek Lovley in 1987 in sand sediment from the Potomac River in Washington D.C. The first strain was deemed strain GS-15.

Metabolic Mechanisms (Newly Added)
For quite some time, it was thought that Geobacter species lacked c-cytochromes that can be utilized to reduce metal ions, hence it was assumed that they required direct physical contact in order to use metal ions as terminal electron acceptors (TEAs). The discovery of the highly conductive pili in Geobacter species, and the proposal of using them as biological nano-wires further strengthened this view. Nevertheless, recent discoveries have revealed that many Geobacter species, such as G. uraniireducens, not only do not possess highly conductive pili, but also do not need direct physical contact in order to utilize the metal ions as TEAs, suggesting that there is a great variety of extracellular electron transport mechanisms among the Geobacter species. For example, one other way of transporting electrons is via a quinone-mediated electron shuttle, which is observed in Geobacter sulfurreducens.

Another observed metabolic phenomenon is the cooperation between Geobacter species, in which several species cooperate in metabolizing a mixture of chemicals that neither could process alone. Provided with ethanol and sodium fumarate, G. metallireducens broke down the ethanol, generating an excess of electrons that were passed to G. sulfurreducens via "nanowires" grown between them, enabling G. sulfurreducens to break down the fumarate ions. The nanowires are made of proteins with metal-like conductivity.

Biodegradation and bioremediation
Geobacter's ability to consume oil-based pollutants and radioactive material with carbon dioxide as waste byproduct has been used in environmental clean-up for underground petroleum spills and for the precipitation of uranium out of groundwater. Geobacter degrade the material by creating electrically conductive pili between itself and the pollutant material, using it as an electron source.

Microbial biodegradation of recalcitrant organic pollutants is of great environmental significance and involves intriguing novel biochemical reactions. In particular, hydrocarbons and halogenated compounds have long been doubted to be anaerobically degradable, but the isolation of hitherto unknown anaerobic hydrocarbon-degrading and reductively dehalogenating bacteria documented these processes in nature. Novel biochemical reactions were discovered, enabling the respective metabolic pathways, but progress in the molecular understanding of these bacteria was slowed by the absence of genetic systems for most of them. However, several complete genome sequences later became available for such bacteria. The genome of the hydrocarbon degrading and iron-reducing species G. metallireducens (accession nr. NC_007517) was determined in 2008. The genome revealed the presence of genes for reductive dehalogenases, suggesting a wide dehalogenating spectrum. Moreover, genome sequences provided insights into the evolution of reductive dehalogenation and differing strategies for niche adaptation.

Vengeanceknight (talk) 22:34, 13 November 2017 (UTC)