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Discovery of Legionella clemsonensis:

The specific strain for Legionella clemsonensis is D5610 (Palmer, et al 2016). It was named in honor of the research group of students from the Clemson’s Creative Inquiry (Colmenares 2016). Legionella clemsonensis was first isolated in 2006 from the bronchial wash of a patient diagnosed with pneumonia (Palmer, et al 2016). Bronchial washing is part of a bronchoscopy procedure (Palmer, et al 2016). After being isolated and stored, strain D5610 was acquired by The Center for Disease Control and Prevention (CDC) (Colmenares 2016). CDC sent 68 strains of Legionella, including strain D5610 for students to analyze at Clemson University (Colmenares 2016). These students first sequenced two genes to identify if they were a match to anything in the database. Results showed one of the strains that was sequenced was not a match. After, it was double checked by a second wave of students along with their assistant professor working together to ensure that Legionella clemsonensis was different from all of the other Legionella strains. When isolated it was shown to be very similar to bacteria genus Legionella. It was identified as Legionella based on sequencing, cellular fatty acid analysis, biochemical reactions, and biofilm characterization (Palmer, et al 2016). The results showed that strain Legionella clemsonensis was closely related to L. lansingensis (Colmenares 2016). For a physical characterization of Legionella clemsonensis, it was streaked for a single colony isolation using a charcoal yeast extract agar and required cysteine for primary isolation (Palmer, et al 2016). No growth was shown, which was typical of Legionella strains. A feature that sets Legionella clemsonensis apart was that under ultraviolet light, Legionella clemsonensis was fluorescent green (Palmer, et al 2016). This is different from other Legionella strains because they usually show up as fluorescent blue, red or yellow. Autofluorescence was assessed by attaching bacteria to coverslips overnight, fixing them in paraformaldehyde and imaging under UV laser on a Leica SP8X multiphoton spectral confocal microscope (Palmer, et al 2016). Another characteristic that was found about Legionella clemsonensis was that it had a single, polar flagella (Palmer, et al 2016). Researchers also used fatty acid methyl ester analyses to distinguish differences between the Legionella strains (Palmer, et al 2016). Cellular fatty acid composition was analyzed using a Sherlock microbial Identification System (Midi, Newark).

WYGD...

Although this organism is fairly new, people should care about Legionella colemsonensis because there is a high chance that this strain is pathogenic. Legionella species are also commonly known to cause pneumonia (Palmer, et al 2016). Currently, the there is not a lot of information about this bacteria. It's metabolism process is still being investigated. One major attribute that sets this strain from other Legionella strains is that it is fluorescent green under ultra violet light (Palmer, et al 2016). Researchers are not sure why or what it means but it is the first strain of Legionella to display that particular color.

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Clinton Colmenares. "Bacteria strain named by Clemson students." Newsstand | Clemson University News and Stories, South Carolina. Clemson newsstand media release, 18 Oct. 2016. Web. 01 Apr. 2017 My partner is Emily Tran this is the link to her sandbox.