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This is done by the enzyme creatine phosphokinase which transduces energy from the transport molecule of phosphocreatine to the useful molecule for contraction demands, ATP, an action performed by ATPase in the myofibril. The resulting creatine product acts as a signal molecule indicating myofibril contraction and diffuses in the opposite direction of phosphocreatine, back towards the mitochondrial intermembrane space where it can be rephosphorylated by creatine phosphokinase.

Creatine supplementation is an effective method of increasing an athlete's power output. Once the molecule is ingested it accumulates in muscle cells where it can be phosphorylated, increasing the pool of phosphocreatine available for contractile power before it is depleted and other energy systems must be used. The result is increased performance in short-term high intensity activities such as sprinting or weightlifting.