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Epitope binning is a competitive immunoassay used to characterize and then sort a library of monoclonal antibodies against a target protein. Antibodies against a similar target are tested against all other antibodies in the library in a pairwise fashion to see if an antibodies block one another’s binding to the epitope of an antigen. After each antibody has a profile created against all of the other antibodies in the library, a competitive blocking profile is created for each antibody relative to the others in the library. Closely related binning profiles indicate that the antibodies have the same or a closely related epitope and are “binned” together [1]. Epitope Binning is referenced in the literature under different names, “epitope mapping,” epitope characterization, etc. Regardless of the naming, epitope binning is prevalent in pharma. Epitope Binning is used in the discovery and development of new therapeutics, vaccines, and diagnostics [1]–[4].

Methods for epitope mapping Several methods are available for epitope binning including Surface Plasmon Resonance (SPR) [2]–[4], BLI [4], and ELISA [1], [5].

References [1]	P. L. Miller, R. L. Wolfert, and G. Diedrich, “Epitope binning of murine monoclonal antibodies by a multiplexed pairing assay,” J. Immunol. Methods, vol. 365, no. 1–2, pp. 118–125, Feb. 2011. [2]	Y. N. Abdiche, K. C. Lindquist, D. M. Stone, A. Rajpal, and J. Pons, “Label-free epitope binning assays of monoclonal antibodies enable the identification of antigen heterogeneity,” J. Immunol. Methods, vol. 382, no. 1–2, pp. 101–116, Aug. 2012. [3]	Y. N. Abdiche, D. S. Malashock, and J. Pons, “Probing the binding mechanism and affinity of tanezumab, a recombinant humanized anti-NGF monoclonal antibody, using a repertoire of biosensors,” Protein Sci. Publ. Protein Soc., vol. 17, no. 8, pp. 1326–1335, Aug. 2008. [4]	Y. N. Abdiche, D. S. Malashock, A. Pinkerton, and J. Pons, “Exploring blocking assays using Octet, ProteOn, and Biacore biosensors,” Anal. Biochem., vol. 386, no. 2, pp. 172–180, Mar. 2009. [5]	B. D. Brooks, A. E. Albertson, J. A. Jones, J. O. Speare, and R. V. Lewis, “Efficient screening of high-signal and low-background antibody pairs in the bio-bar code assay using prion protein as the target,” Anal. Biochem., vol. 382, no. 1, pp. 60–62, Nov. 2008.