User:Wbhagen

Wayne Hagen wbhagen@hotmail.com

EDUCATION: University of California, Irvine					 	August 2005 Bachelor of Science, Biology •	Undergraduate Research Opportunity Program Fellowship; 2003-04

LABORATORY SKILLS: •	PCR •	RT-PCR •	qPCR •	Sub-cloning •	Mass Spectrometry (MS) •	Embedding and Sectioning tissue for histology •	DNA Isolation •	Restriction Endonuclease digestion/ligation •	Mammalian Tissue Culture •	Transfection •	Mouse Dissection •	RNA Purification •	cDNA Synthesis •	Western and Southern Blotting •	Transformation

EXPERIENCE: Staff Research Associate II					University of California, Irvine October 2005 – December 2006			Irvine, CA Description: I was primarily responsible for the relocation and setup of 2 new labs at the University of California, Irvine. Ordering and purchasing of supplies and equipment. My essential job responsibilities were to establish and maintain mouse colonies (approximately 12 different lines), genotyping, experiments and record keeping, organization of the lab, and general laboratory maintenance. I assisted with some real time-PCR experiments in the lab. Also I performed many of the same functions below.

Lab Assistant							University of California, Irvine Sept 2001 – August 2005				Irvine, CA Description: I have extensive knowledge of general laboratory practices, expectations, safety and procedures. My primary duties in the lab consisted of washing laboratory glassware, autoclaving, and maintain the mouse colony (approximately 3 different lines). I was the lead undergraduate and was heavily involved with teaching and training new students in basic laboratory procedures. In the lab I helped with experiments and did some personal projects for course credit through the University of California, Irvine.

Intramural Research Traineeship Award				NIH/NHGRI July 24th 2003 – August 29th 2003			Bethesda, MD Description I spent the summer working in Dr. Francis Collins’ laboratory at the National Human Genome Research Institute in Bethesda. Under the stewardship of Dr. Maria Eriksson, we were studying how a single nucleotide polymorphism in LMNA causes Hutchinson-Gilford progeria syndrome. Also I performed high throughput screening and genotyping of Single Nucleotide Polymorphisms via mass spectrometry. Some of the functions I performed were Sub-cloning, RNA isolation, cDNA synthesis and reverse transcriptase-PCR

COMPUTER SKILLS: Knowledge in MS Word, MS Excel, PowerPoint, Sequencher, Networking, Photoshop, Illustrator. Platforms: Macintosh, PC

PUBLICATIONS: Peng-Chen, C.; Dudley, S.; Hagen, W; Dizon; D.; Paxton, L.; Reichow, D.; Song-Roo, Y.; Kan, Y.; Arnheim, N.; Liskay, M.R.; Lipkin, S.M. Contributions by MutL Homologues Mlh3 and Pms2 to DNA Mismatch Repair and Tumor Suppression in the Mouse. Cancer Research 2005; 65: (19). October 1st, 2005

Varga, R.; Erdos, M.R.; Virmani, R.; Kolodgie, F.D.; Avallone, H.; Hagen, W.; Nabel, E.G.; Collins, F.S.; & Eriksson, M. Characterization of a mouse model for Hutchinson-Gilford Progeria Syndrome. The American Society of Human Genetics 54th Annual meeting, Toronto, October 26-30, 2004