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Small Integral Membrane Protein 8 (SMIM8) is also known as C6orf162 or DC18. It has 97 amino acids and weighs 11059 Daltons. The domain containing the 15th to 96th amino acids belongs to the Pfam DUF4500, which means “Domain of Unknown Function”. There are two isoforms for SMIM8 protein with 76 and 58 amino acids respectively.

The gene that encodes this protein is located at chromosome 6q15 and has a total of 2438 nucleotides. There are 15 promoters and enhancers that regulates the expression of SMIM8 gene.

SMIM8 is ubiquitously expressed in brain, endometrium, thyroid and many other tissues. It is predicted as intracellular protein and membrane protein. Orthologs for SMIM8 gene were found in mouse, zebrafish, chicken and lizard.

RNA Structure
There are 2438 nucleotides and 4 exons in SMIM8 mRNA sequence. Exon 1 contains nucleotides 1-45, exon 2 contains nucleotides 46-66, exon 3 contains nucleotides 67-224, and exon 4 contains nucleotides 225-2438. There is a transmembrane domain in the protein located at nucleotide 231-299.

The transmembrane region in human protein is almost conserved in Danio rerio, with possible mutations in several amino acids.

There are also two highly conserved regions upstream of the transmembrane region, which could be important for SMIM8 to have proper function.

RNA Variants
There are several somatic confirmed variants that related to tumor in human body.

Secondary Structure
The secondary structure is generated using RNAfold web server

Protein Expression
SMIM8 is expressed in several tissues in human body.

Protein Interation
SMIM8 interacts with TNIP2, RANBP6, C6orf163, AP1S2, MRPS15, TMEM107, FBXO8, HMCES, FAM162A, and PIWIL2