User:Will-moore-dundee

Warm PBS &amp; Media to 37'C
Defrost Trypsin.

Wash cells in PBS
Rinse cells 3x in warm PBS.

Add trypsin: 5   ml
Default Value = 5Add trypsin, then remove and add another few ml. Remove and incubate, with shaking, till cells start to come off the dish.

Resuspend in media: 5   ml
Default Value = 10This will stop the trypin reaction.

Cell Line
Default Value = HeLa

Description
Notes about the cells:ie Are they stably transfected?Where they came from, etc.

Passage Number passages
The number of times the cells have been passaged since they started growing.

Plate format: 6-well plate
Default Value = 24-well plate