User:Yates9/molecular biology techniques

Learning Neuroscience

 * Creating a Wikibook Notes
 * Books for experts


 * Imaging Technology
 * Quantum dot semiconductor nano-crystal that is sensitive to light, usually UV. Less susceptible to photobleaching, can measure Ph surrounding it.
 * FRET imaging Förster resonance energy transfer: technique to visualize interactions between especially marked molecules, which emit florescence as part of interaction


 * Entries following need serious review.. many misclassified etc..


 * Arabidopsis and Botany Protocols
 * Protoplast Isolation Protocol unfound


 * DNA
 * Molecular cloning (DNA Cloning) isolating a defined DNA sequence and obtaining multiple copies of it in vitro
 * Gel Electrophoresis (DNA also Agarose) technique used for the separation of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein molecules using an electric field applied to a gel matrix
 * DNA Thermodynamics, Hybridization
 * Membrane Hybridization Screening of Libraries ?
 * DNA sequencing and Maxam-Gilbert Sequencing
 * Molecular cloning or Gene Cloning
 * Restriction Enzyme (also Digestion)
 * DNA Transformation - Contains History of DNA
 * Electrophoretic Mobility Shift Assay


 * General
 * Gel Electrophoresis
 * Liquid Scintillation Counting work with Beta particles emitted from the sample transfer energy to the solvent molecules, which in turn transfer their energy to the fluors; the excited fluor molecules dissipate the energy by emitting light
 * Autoradiograph with Phosphorimaging just reference
 * Non-radioactive detection methods and tracers.
 * HPLC - High Performance Liquid Chromotography


 * Immunoprecipitation
 * Immunoprecipitation Background
 * Immunoprecipitation Protocol


 * Mass Spectrometry
 * Mass Spectrometery
 * Edman Degradation
 * Electrospray MS


 * Microarray
 * Protein Microarray Theory


 * PCR - Polymerase Chain Reaction
 * Theory and Development of PCR
 * Avoiding PCR Contamination
 * PCR Primer Design Guidelines and Software

Protein Techniques
 * Protein Identification
 * Protein Purification
 * Protein Separation
 * Western Blot
 * Immunoprecipitation IP
 * Proteomics Methods


 * Recombinant Protein Expression
 * Recombinant Protein Expression


 * RNAi and SiRNA
 * SiRNA Transfection Protocol


 * SDS-PAGE Gel Electrophoresis
 * SDS-PAGE Gel Electrophoresis Background Theory


 * Trypsin Digestion (In Gel Trypsin Digestion)
 * In Gel Trypsin Digestion Theory


 * Transgenic Plants and Animals
 * Transgenic Animals


 * Western Blot
 * Western Blot Background Theory
 * Western Blot Troubleshooting
 * Western Blot Protocol


 * X-Ray Crystallography
 * X-ray Crystallography


 * Nucleic acid fractionation


 * Cloning
 * Polymerase chain reaction
 * Vectors
 * Plasmid
 * Promoters


 * Gene (DNA) Sequencing
 * Polymerase chain reaction
 * contigs


 * Gene Expression
 * Probes, Hybridization
 * Microarray


 * Electrophoretic separation of nucleic acid


 * Detection of genes:
 * DNA: Southern blotting; inSitu hybridization; FISH Technique
 * RNA: Northern blotting
 * Protein: Western blotting, immunohistochemistry