User:Yuqingli1015/P1-Derived Artificial Chromosome/Yestothesun Peer Review

General info

 * Whose work are you reviewing?

Yuqingli1015


 * Link to draft you're reviewing
 * https://en.wikipedia.org/wiki/User:Yuqingli1015/P1-Derived_Artificial_Chromosome?veaction=edit&preload=Template%3ADashboard.wikiedu.org_draft_template


 * Link to the current version of the article (if it exists)
 * P1-derived artificial chromosome

Evaluate the drafted changes
(Compose a detailed peer review here, considering each of the key aspects listed above if it is relevant. Consider the guiding questions, and check out the examples of what feedback looks like.)

Lead


 * The lead looks good when your added edit! It is concise and mentions the other sections you plan to add to the article.
 * Does PAC stand for P1-derived artificial chromosome specifically or just P (any number) derived artificial chromosome?

History of PAC


 * Are P1 and P2 just other types of phages?
 * The current article mentions "P1 was developed as a cloning vector by Nat Sternberg" in the lead section but I didn't see this name mentioned in your article's history section. Not sure which one is correct but just a heads-up to make sure they match in terms of who discovered it etc.
 * Add what year this was discovered if possible

Construction


 * I'm unsure of what the first sentence says. How are the loxP sites helpful for the product?
 * "which allows plasmids entering the cells" --> "which allows plasmids to enter [bacterial] cells"
 * What is P1 lytic replicon? Is it a sequence that can be added in the construct? Since this is probably quite unique and won't have it's own Wikipedia page on it, it would be helpful to quickly explain what this is in a sentence. Also, a citation should be included for where you found this could be used for generating high gene expression levels.
 * If you wanted to add more, you could include a few sentences about how the chromosome is constructed generally by linking to a Wikipedia page that might explain how artificial chromosomes are made. Also maybe how genes of interest are added into these artificial chromosomes.

Comparison with other artificial chromosomes


 * It would be helpful to add how many kb PAC can hold versus the yeast artificial chromosome you compared it to.

Application


 * I think it's a great list! Can this chromosome be transformed into other bacteria as well? Or any other types of cells?

General Comments


 * I think the sections you plan to add are great and in a logical order.
 * If possible, adding a diagram of an artificial chromosome and it being inserted into a genome could be helpful. Or a diagram of this artificial chromosome and the specific sequences (like loxP sites) that it has.
 * Are there any drawbacks to this using this chromosome? Is it commonly used and why/why not?

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