User:Zhannaty/sandbox

Ion exchange chromatography technique is used in separation of products of asymmetric hydrolysis of amino acids. Addition of ethanol separates free L-amino acids from unhydrolyzed D-derivative. However, some amino acids are soluble in alcohol so ion exchange chromatography should be used to separate them. For instance cation exchange chromatography is used after enzymatic resolution of isovaline. Free L-amino acid are retained by the column and unhydrolyzed derivative passes through it.