User:Zhen2901/User:AssertiveClatter/Heterologous expression/Zhen2901 Peer Review

P1:

Instead of hyperlinking the Wikipedia page for "molecular cloning" on "Recombinant DNA technology", I would rephrase the sentence as follows, "insertion of the gene in the heterologous host is performed by recombinant DNA technology, including molecular cloning, etc. etc.", then link the "molecular cloning" term with its respective link. Cloning is an example of DNA technology, not the term itself, which is how it's currently read with the way it linked now.

Furthermore, where the authors write "it provides a facile avenue to efficiently express and experiment with combinations of genes and mutants that do not naturally occur", it seems redundant to the first sentence. Either combine the two definitions or remove one of them for conciseness.

P2:

Incorrect footnote annotations where the authors discussed techniques to isolate and incorporate genes into host.

P3:

The author writes "the host is called the expression system" and links the term "expression system" with "gene expression". This was a little confusing because they're saying the host is a system. I'm not sure if thats what they intended for the audience to grasp, but as of now, that's how its read and annotated. Maybe add an additional sentence or two to clarify this.

P4:

This paragraph is a repeat to the first paragraph, with the addition of stating specific examples of what host organisms can be. I think the authors can combine this with the previous paragraph to make it more concise.

P5:

"This technique can be used for both short and long term transfectants". This sentence can be removed because in P2, the authors already talked about how heterologous expression can be for short and/or long term modifications

P6:

"it is also effective with almost any tissue type, and has displayed high levels of gene delivery with an increase in distribution of cells expressing the DNA.". This sentence is again just rewording what the definition of homologous expression, which is overexpression of cells expression the DNA, which we get in P1 and the methods already.

P8:

"Genes are subjected to heterologous expression often to study specific protein interactions. E. coli, yeast (S. cerevisiae, P. pastoris), immortalized mammalian cells, and amphibian oocytes (i.e. unfertilized eggs) are commonly for studies that require heterologous expression." This sentence has been copied from the previous section, and was again copied in the latter. You should only keep it once.

Overall:

I thought the writing was a bit verbose and redundant. The article was very informative, but at the same time, there were moments where I felt distracted/lost as to how your methods and limitations sections directly correlated back to the discussion of heterologous expression. I felt like the methods section was primarily written as background information; however, if there exists a wikipedia page for it already, maybe it would've been easier them to just link pages to keywords. The author also mentioned that there is applications both in-vitro and in-vivo, but only one is mentioned in the end (the in-vitro drug development). I think with the approach of this topic, the focus should be more on the applications of heterologous expression and what they are, and less about the types of methods used to determine it (since that currently makes up more than half the writing). Nevertheless, the information and sources they provided were definitely reliable, neutral, and relevant to the study!

General info

 * Whose work are you reviewing?

Reika222, Emilyyao01, AssertiveClatter, Ddenoo


 * Link to draft you're reviewing
 * User:AssertiveClatter/Heterologous expression


 * Link to the current version of the article (if it exists)
 * Heterologous expression

Evaluate the drafted changes
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