User talk:Bedrosmi/sandbox

Peer Review by Irena Djordjevic
Good start to your article. Overall, try to look for information regarding your fungi in books, as I see you only have journals and websites that seem to be listed in your references - books may allow you to find some general important information that journal article don't bother mentioning. Irena Djordjevic (talk) 18:17, 27 October 2016 (UTC)
 * Try to look for some information about the history of Fonsecaea compacta (ex: when it was first isolated, by who, what it was named at the time etc.) - it might be a good idea to look into some books for this information
 * Try to find some more information regarding the habitat of your fungi. Is it a soil fungi? Water-borne? etc. The geographical information is very well presented, but it would be interesting to know what it grows on in these areas.
 * While looking for information in books, try to find information about F. compacta nutrient usage (ex: what is it's ideal carbon source, ideal nitrogen source etc.). My fungi is a soil fungi and I was able to find this information in the book "Compendium of Soil Fungi" . If it turns out that your fungi is also a soil fungi, it might be interesting to look in there. Otherwise, look for similar books related to your fungi.
 * The division of the "Disease" sub heading into three parts is great, allows for easy reading! Might be good to the same in other sections as you come across more information.
 * Would be great to explain what you mean by "its morphological forms" in the Growth and Morphology subheading - are these different forms associated with different periods of growth? Are they all present at the same time? What makes Cladosporium-like growth form different from Rhinocladiella-like growth form? etc.
 * Great description of the physiological appearance of the fungi.
 * Great description of the confusion regarding taxonomy/classification of F. compacta, great use of references here as well.
 * Were you able to find who gave the fungi its binomial name, if yes, include that where you listed the binomial name.
 * Overall, try to find more information to include in the parts of your article describing the fungi, as opposed to the part of your article describing the disease caused by the fungi.

Peer Review by Stefaniemogen
Hi Bedrosmi,

I noticed that your fungus was also an agent of chromoblastomycosis, as mine is. That's why I thought it would be helpful if I review your article, and I can relay some information that I learned about chromoblastomycosis as well.

I think you have very great points in the classification section, so maybe you could format the section by starting with in what ways F. compactum is really similar physiologically and morphologically to F. pedrosoi and why they used to be considered the same species. And then talk about how scientists discovered that they had enough genetic differences to be categorized as different species. Since they have base changes in 48 positions, does that mean they were the same species at one point and one evolved genetic changes to become a separate species? Try and find out if F. pedrosoi gave rise to F. compactum or vice versa - and if those changes contributed to better infect humans and more pathogenic? Although, I searched up your fungus in the articles I used for my article and I found something interesting. It was from a relatively recent review article (from 2009 by Queiroz-Telles et al.), saying that the taxonomic naming of F. compacta isn't valid anymore and is just considered a morphological variant of F. pedrosoi, which you may want to add in your article. Also - I'm a bit confused as to what you're hinting at by the fact that the two species are polymorphic - if they form different arrangements of conidia, does that mean they look like the same species? I suggest you elaborate on that point. My last comment about your classification section is that you might not want to incorporate your last point, because it seems like it's stating a position/belief - that a new taxonomic study should be conducted - and it doesn't really fit with the Wikipedia vibe.

One comment I had along the way was why your fungus switches between F. compacta and F. compactum - not sure if it was a spelling mistake or if they are actually different forms of the same species (if so, then elaborate on the naming). Following are my comments about your growth and morphology section. Can you elaborate on what standard conditions the most stable form of the fungus is found (ex. temperature, culture method, nutrient concentration)? Also, I noticed that you described the conidiophores, so I know F. compacta produces asexual spores - did you find anything about its sexual state, if it has one?

Physiology section: What function do the D1/D2 sequences play? Do these sequences contribute to their morphological and physiological similarity somehow? If RFLP couldn't find variation between the species, how does it contradict with your previous statement saying there are base changes in 48 positions - find differences between methods used? What roles do the glycolipids in F. compacta play in pathogenesis? I know you said little is known about the composition of the glycolipids, but does it seem that there are glycolipid differences that cause differential severity of pathogenesis?

Habitat and ecology: Only one comment for this one - you might want to find out if F. compacta are biotrophs (facultative or obligate) or saprotrophs.

Disease section: I'll have to correct you on the names of other fungi that cause chromoblastomycosis - Cladosporium carrion was actually the old name for the fungus, it is now called Cladophialophora carrionii, which is coincidentally my fungus! :) Also - I know it's especially difficult to find information on your fungus, since it's such a rare agent of chromoblastomycosis, but I'm not 100% sure if you should use your first reference about the case studies in Sri Lanka since it's a primary article - you should check with Ian from Wiki Ed. You should also elaborate more on why chromoblastomycosis is more predominant in males than females, and why it's rarely seen in adolescence.

Transmission: You noted that chromoblastomycosis often occurs in immunosuppressed patients - why is this? Is it because there is something in the immune system that is helpful in preventing infection?

Treatment: You listed a lot of treatments that are used, but what are their efficiencies in successfully curing the infection?

Miscellaneous comments: You should see if you can find out more about diagnosis of chromoblastomycosis that is caused by F. compacta - is it diagnosed by culture, microscopy, histology, PCR etc. You mentioned that F. compacta is polymorphic - what evolutionary purpose does this serve? Does it change forms when it penetrates into the human host? You also mentioned that its morphology shows black cells, which I'm assuming is due to the presence of melanin - does melanin have a function in chromoblastomycosis infection? Last comment - you should cite your references using the template instead of autociting inline (I did the latter at first, realized my mistake, and cited them using the references template which Dr. Scott wants us to do)

Overall, I can tell you did quite a lot of research, since it is difficult finding information on F. compacta from what I found. Hope my comments helped you and good luck - can't wait to see the final product!

References: Queiroz-Telles, Flavio; Esterre, Phillippe; Perez-Blanco, Maigualida; Vitale, Roxana G.; Salgado, Claudio Guedes; Bonifaz, Alexandro (2009). "Chromoblastomycosis: an overview of clinical manifestations, diagnosis and treatment". Medical Mycology. 47 (1): 3–15. doi:10.1080/13693780802538001

Stefaniemogen (talk) 23:36, 27 October 2016 (UTC)