User talk:Bwongcha/sandbox

For the EcoRI article, I would like to propose a couple improvements. In the introduction, it says that EcoRI "creates 4 nucleotide sticky ends". This may be misleading in a couple ways. From that phrase, I couldn't tell whether the sticky ends were created on one of the strands in four different locations, or if it meant the length of the sticky ends was 4 base pairs. It should be made clear that EcoRI makes two cuts, each cut creating a sticky end. It should go on to describe what a sticky end actually is. The "5' end overhangs of AATT" was a little vague, as well. Maybe here, it could be specified that the cut was made between the G and the A, as shown in the figure.

In the figure of the EcoRI recognition site, it might be ideal to include the 5' and 3' ends.

Maybe also explain that restriction enzymes tend to be palindromic with their complementary sequences.

It may be useful to include an example of EcoRI being used for genetic deletion from, say, a target plasmid.

Bwongcha (talk) 11:08, 13 February 2016 (UTC)