User talk:Dylan L/sandbox

Treadmilling

Critique:

Lead:

First two sentences need citations. There is also no mention of the biological significance of treadmilling other than "seemingly "moving"". Why would cells want to move this way (food, response to external stimuli etc.) According to one paper, the biological significance of treadmilling in vivo is not well characterized [1]. However, this article was published in 1980 and significant research on the topic has taken place since then and must continue to be researched.

Dynamics of the filament:

First statement requires citations, along with the entire paragraph on polymerization dynamics. Poor title as it is ambiguous to what filaments the author is discussing.

Critical concentration:

Really good information. However, I feel as if the critical concentration is described twice, once under dynamics of the filament and now here. Maybe this should be rearranged, as I feel as if this information could be even more condensed.

Steady-state treadmilling:

Citations required. Any biological functions of steady-state treadmilling (movement)? What if both ends are growing, does that change the implicit function of the filament over steady-state treadmilling? Roles in mitosis? These are all important concepts that are not answered in this article and could be researched and expanded upon. Citation #3 describes the advantages to having this type of polymerization; Nucleation limits where the filaments can polymerize, thus by having the filament subunits diffuse around the cytoplasm, the cell can undergoes morphological changes in response to an internal or external stimulus.

What I would like to improve:

I would break the "Dynamics of the filament" section down into two subsections, one for microfilaments and one for microtubules and describe the chemical and biological differences. I do not think "Detailed process" is a good title. No information on how critical concentration or other molecules affect polymerization of actin or tubulin and subsequently treadmilling. For example, actin polymerization can be regulated by profilin and cofilin [2] This is a simple but necessary addition to this article that are linked to other wikipedia articles where users could find more information. Stathmin and catstrophe of microtubules could also be discussed.

Besides these constructive critiques, this article did a lot of things well. The writer was neutral in his discussion, and plagiarism is not evident. The author includes reliable references, however, his use of in-text citations is on the conservative side. It was also written in a way that a general audience would be able to understand it. The biggest thing that this article is missing is clear structure as, again, many subheadings are missing and I feel as if critical concentration is needlessly described twice. The main thing I would like to improve upon in this article is expanding on the differences between actin and tubulin treadmilling along with the biological significance of this action.

Cell movement Molecular biology Essay.svg This is a user sandbox of Dylan L. A user sandbox is a subpage of the user's user page. It serves as a testing spot and page development space for the user and is not an encyclopedia article. Get Help Jump up ^ "Implication of Treadmilling for the Stability and Polarity of Actin and Tubulin Polymers In Vivo". The Journal of Cell Biology. 86. Jump up ^ Remedios, C. G. Dos; Chhabra, D.; Kekic, M.; Dedova, I. V.; Tsubakihara, M.; Berry, D. A.; Nosworthy, N. J. (2003-04-01). "Actin Binding Proteins: Regulation of Cytoskeletal Microfilaments". Physiological Reviews. 83 (2): 433–473. doi:10.1152/physrev.00026.2002. ISSN 0031-9333. PMID 12663865.Dylan L (talk) 15:50, 26 October 2016 (UTC)