User talk:Gpsrivastava

Advancement in high-throughput technologies has transformed molecular biology from studying single gene to characterizing entire genome. To address the challenges in genome-scale design for these technologies, we developed PRIMEGENS (PRIMEr Design Using GEN Specific Fragments) for PCR and probe design. We applied PRIMEGENS in DNA synthesis, qRT-PCR (gene expression profiling), 454 sequencing for methylated genome, oligo design, Taqman probe design, and array-based sequence capture. PRIMEGENS has a significant edge over other tools for large-scale design with high precisions and in automated fashions. It has >1000 downloads for academic users, >50 paper citations, and >4400 google hits with the “PRIMEGENS” keyword. We have a broad user base and numerous collaborations around the world. The following are some key capacities of PRIMEGENS in genome-scale design:

(1) Select gene-specific fragments and then design primer pairs for PCR amplifications.

(2) Design gene-specific probes using in oligo arrays and pull-down arrays

(3) Design primers/probes for bisulphate-treated sequences.

PRIMEGENSv2.0.5 onward releases use configuration file to implement various types of primer design algorithms like gene-specific primer design, probe-specific primer design, fragment-specific primer design and sequence-specific primer design. It also supports various types of user input data format to allow users to use it in a more efficient way. For more information, please follow "README" file, which comes alongwith the program.