User talk:Jennaconnolly/Locked nucleic acid/Basketballwatersportblue Peer Review

Peer review

Peer Review By Selena Bazzi of Group 8 (Jennaconnolly; Locked nucleic acid)

Content

The introductory section is brief and provides the basic information necessary. The introduction mentions how the enhancement of stacking and backbone pre-organization significantly increases hybridization properties. Perhaps you can further elaborate on the further impact of the increase in hybridization properties. I think it would be great to in fact write a section on the page about the implications of high-affinity hybridization. Check out this paper further as it mentions the implications: https://pubs.acs.org/doi/10.1021/bi025907j

Synthesis section could use some expansion. How was synthesis analyzed? Perhaps include the figure from the linked source? It could be useful information that would provide the reader with the knowledge it was synthesized starting from uridine, etc.

I also like that a link page for defining RNA nucleotide is provided. It is also useful to have it for oligonucleotides as well. The highlighted examples appear to be appropriate as they are linked to relevant pages. Also the content does not seem duplicative of any other content already on Wiki.

Figures

The figure is informative in the sense that it provides a visual of the chemical structure of the monomer. There are two figures, one that shows the chemical structure of the LNA monomer and another that shows the conformational structure of it in B-D configuration. It is also aligned and easy to read. Would ChemDraw have to be referenced? I am not sure about that and would suggest looking into that.

References

I only see 6 references. Be sure to include at least 10! The references used however are inclusive and relevant.

Overall Presentation

Overall, the paper is really short and could use much more expansion. I really think it would be useful to develop a section focusing on the implications of the high-affinity hybridization. I linked the paper above that would be really useful in carving out that section. The introduction is straightforward. The therapeutic applications section is also very short and could use further expansion. For instance, what is the usefulness of the ability to design shorter primers? I think expanding on this it could really help provide non-experts a more comprehensive understanding of LNA. The content that is provided seems to belong on the page and the information is provided from a neutral approach. I checked a few links and they seem to work and reflect reliable information. Though the paper is short, the wording is clear and easy to read. I really do think though the paper needs much more expansion on LNA. If there is not much more to report about it, perhaps include possible studies in progress that seek to tell us more about LNA. Also, is there anything researchers are still trying to learn more about?

by: Basketballwatersportblue

Basketballwatersportblue (talk) 17:37, 1 December 2020 (UTC)