User talk:Jogalous

endogenous Plastic Somatic cells or ePS cells, are somatic cells that can differentiate into a variety of cell types representative of all the three germ layers (endoderm, ectoderm and mesoderm). ePS cells have been differentiated to adipocytes (fat cells ), osteocytes (bone), beating cardiomyocytes (heart), pancreas and milk producing alveolar cells. ePS cells were first discovered at UCSF in Dr. Thea Tlsty's laboratory. They were first isolated from the mammary gland tissue by enrichment for cells expressing high levels of cell surface protein CD73 and low/no expression of CD90 (CD73+/CD90-) using fluorescent activated cell sorting (FACS). The Tlsty laboratory continues to isolate ePS cells from other tissues in both mice and humans.

Characteristics

Isolation

ePS cells were first isolated from primary mammary gland tissue by FACS selection of CD73+/CD90- cell fraction. Once CD73+/CD90- cells are obtained, they are cultured on feeder or feeder-free conditions and after several days, activated ePS colonies proliferate and become large colonies visible under the microscope. CD73+/CD90- cells are estimated to compose ~3-4% of total mammary epithelial cells while ePS cells are ~0.15% of total.

Plasticity

ePS cells are unique from other pluripotent/multipotent cells such as induced Pluripotent Stem Cells (iPSCs), embryonic stem cells (ESCs) or mesenchymal stem cells (MSCs) in that they are naturally in a latent or dormant state until they become activated by environmental signals to display pluripotent/multipotent characteristics.During activation, ePS cells increase their expression of pluripotency genes SOX2, OCT3/4 and NANOG. Once pluripotency genes are upregulated, ePS cells gain the ability to change into different types of tissues. ePS cells have been directed to differentiate into adipocytes, osteocytes, mammary lineage cells - myoepithelial, luminal, bipotent and milk producing acini cells, pancreatic cells, neuronal progenitors and beating cardiomyocytes. ePS cell plasticity is short lived with a maximum population doubling of ~25-30.

Morphology

ePS cells have epithelial phenotype during early passages. Late passage ePS cells are more mesenchymal and don't expand as a colony. cellThese cells have similar characteristics to embryonic stem cells (ESC) such as ability for self-renewal and differentiation into functional derivatives of all three germ layers - endoderm, mesoderm and ectoderm. ePS cells express pluripotent cell surface markers, SOX2, Oct3/4 and Nanog just like ESC. ePS cells are different from human ESC by exhibiting low amounts of DNMT3B and being mortal as a result of low telomerase activity. ePS cells's ability to differentiate into any cell in the body and still remain mortal makes them very good candidates for cell therapy and regenerative medicine.

Your submission at Articles for creation: User:Jogalous/sandbox (March 11)
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Kevin Rutherford (talk) 22:09, 11 March 2014 (UTC)
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Your submission at Articles for creation: User:Jogalous/sandbox (March 14)
 Your recent article submission to Articles for Creation has been reviewed! Unfortunately, it has not been accepted at this time. Please read the comments left by the reviewer on your submission. You are encouraged to edit the submission to address the issues raised and resubmit when they have been resolved. ''' Thank you for your contributions to Wikipedia! '''
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Kevin Rutherford (talk) 01:27, 14 March 2014 (UTC)
 * You can also get real-time chat help from experienced editors.

Your draft article, User:Jogalous/sandbox


Hello Jogalous. It has been over six months since you last edited your WP:AFC draft article submission, entitled "sandbox".

The page will shortly be deleted. If you plan on editing the page to address the issues raised when it was declined and resubmit it, simply and remove the  or  code. Please note that Articles for Creation is not for indefinite hosting of material deemed unsuitable for the encyclopedia mainspace.

If your submission has already been deleted by the time you get there, and you want to retrieve it, copy this code:, paste it in the edit box at this link , click "Save page", and an administrator will in most cases undelete the submission.

Thanks for your submission to Wikipedia, and happy editing. JMHamo (talk) 15:47, 26 October 2014 (UTC)