User talk:Karismahai

Recent publication on rhinosporidiosis: Ahluwalia, Karvita B., Dhaulakhandi, Dhara B and Garg, Lalit C. 2010. Sequence analysis of 16SrRNA gene in Rhinosporidium seeberi shows similarity to plant chloroplast DNA. Bioinformation 5 (3) : 89-96. The 16S rRNA gene detected recently by these authors shows 99% similarity with 16S rDNA in chloroplasts of land plants. This is a first report of a chloroplast sequence in R. seeberi. A comparison of the 16S rRNA sequence in R. seeberi with 16S rDNA in 11 cyanobacteria confirmed presence of cyanobacterial sequence characteristics in R. seeberi.

Paper also provides information regarding another molecular study on R. seeberi (Fredricks et al 2000. Emerging Infectios Diseases 6:273-282; Herr et al 1999. J. Clinical Microbiology 37:2750-2754), that is based on DNA isolated from infected tissue and not from purified R. seeberi, that does not support the conclusion concerning R. seeberi's relationship with Dermocystidium (Mesomycetozoa). The primers used by Fredricks et al (2000) could not have given the amplicon shown in results, and "data not shown", while in Herr et al's (1999) study, mandatory controls are lacking.