User talk:Mullneree/sandbox

Manual Cell Picking
Micromanipulators used for manual cell picking usually consist of an inverted microscope and micro-pipettes which are moved by motorized mechanical stages. The micropipettes are made of thin glass capillaries linked to an aspiration and dispensation unit. The operator, using a microscope, will select a specific cell, move the micropipette and aspirate the cell through suction. This aspirated liquid, which includes the selected cell is transferred to a collection vessel to be released by dispensation. Micromanipulators allow for controlled separation of selected, living cells from suspension and allows for isolation of prokaryotic cells. This technique of single cell-assay is helpful for bacterial analysis, forensics, and reproductive medicine

Microfluidics
This is a method that will allow isolation of single cels for further downstream analysis or culture. . There are a variety of different microfludic devices fro single-cell separation, most of these devices use at least one of the following microfluidic princples in isolating single cells: Droplet-in-oil based isolation, pneumatic membrane valving, and hydrodynamic cell traps. . Droplet-based microfluidics uses oil-filled channels to hold separated aqueous droplets. Single cells can be contained and isolated from the droplets. An advantage to this method is high throughput of up to several thousand single-cells per second. . Pneumatic membrane valves use pressuriezed air to deflect and elastomer membrane. This membrane shuts a microfluid channel, which allows for digitally opening or closing channels in a microfluidic network. This system requires an operator, typically, and are limited in throughput. Hydrodynamic traps are more passive in a microfludic channel and only allow one cell to enter. To avoid double occupation, the trap size is reduced to the average cell size of the sample at hand. Each of the above techniques is considered a microfluidic lab-on-a-chip technologies for single cell applications; they are designed to serve a particular application which is indicative of why there is little flexibility in upstream sample preparation and downstream analysis methods. Mullneree (talk) 03:43, 2 March 2020 (UTC)