User talk:Rakesh Kacham

Split ratio: Fraction of cells used from completely grown cell culture to subculture the new media. Split ratio is decided based on experience. Poor split ratio results in exhaustion of the essential nutrients and cells will die before completion of their actual incubation period.

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Rakesh Kacham (talk) 18:08, 25 January 2012 (UTC)Split ratio Fraction of cells used from completely grown cell culture to subculture the new media. Split ratio is decided based on experience. Poor split ratio results in exhaustion of the essential nutrients and cells will die before completion of their actual incubation period.