User talk:Scaffolder

Welcome!

Hello, Scaffolder, and welcome to Wikipedia! Thank you for your contributions, especially what you did for DARPin. I hope you like the place and decide to stay. Here are some pages that you might find helpful:
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I hope you enjoy editing here and being a Wikipedian! Please sign your messages on discussion pages using four tildes ( ~ ); this will automatically insert your username and the date. If you need help, check out Questions, ask me on my talk page, or ask your question on this page and then place  before the question. Again, welcome! --ἀνυπόδητος (talk) 15:38, 19 July 2010 (UTC)

Just one hint: Please don't mark your edits as minor unless they are really trivial edits like fixing of typos or grammar. Thanks. --ἀνυπόδητος (talk) 15:38, 19 July 2010 (UTC)

DARPins
Hi Anypodetos, very nice work on the different antibodies/antibodyfragments/scaffolds entries! The "minor" was a newbie error. The last change in the DARPins entry is to make it more clear that it is derived from natural repeat proteins and it's not artificial. The same applies for all other scaffolds like e.g. affibody, anticalins, Affilins, Adnectins, and Avimers which are similarly derived from natural proteins. Scaffolder (talk) 13:51, 27 July 2010 (UTC)


 * They are derived from natural proteins but are artificial (genetically engineered and not occurring in nature in this form) themselves. Does my present version make the distinction clear?
 * Also, are you sure about 14 kDa for the lowest DARPin mass? This is a four-repeat ankyrin and has 13.6 kDa, so three-repeat proteins should be around 10 kDa (3 time 33 amino acids of about 100 Daltons on average). I think I found one of these somewhere, I just can't remember where.
 * Third point: Could you explain the abbreviations Tm, dG and SRP in the article? Thanks, ἀνυπόδητος (talk) 15:15, 27 July 2010 (UTC)


 * Genetically engineered reflects reality well. I copied it to the monobody entry as well.
 * You are of course absolutely right. Yet, which weight to put for proteins which can be 10, 14, 18, 22, 26,.... kDa? I assumed a fair range would be to indicate the weight of the most common sizes, e.g. 4 and 5 repeat proteins
 * The third point should be clarified now


 * Thanks for the good work! I did some copyedit to explain the mass issue; could you check that? Cheers, ἀνυπόδητος (talk) 08:57, 28 July 2010 (UTC)


 * Looks good! Scaffolder (talk) 09:07, 28 July 2010 (UTC)