User talk:Seaveys/sandbox

Considerable research has been funneled into the biological implications relating to the presence of H-DNA in the major breakpoint regions (Mbr)What is a major break point reigon? Is there a way to make that a link to another page? and double-strand-breakpoints (DBS) of certain genes. For example, major points of breakage in the P1 promoter of the c-MYC gene have been found neighboring polypurine mirror-repeat H-DNA forming sequences among other non-B DNA structure forming sequences maybe just a quick sentence about what this does like does it cause mutatuion or illness or inhibit something?. Cases of genetic instability were also observed in the F1 offspring of transgenic mice after incorporation of human H-DNA-forming sequences paired with Z-DNA sequences into their genomes where no instability was previously reported —what instability occurs? Weaker H bonds? non-watson krik pairing?.[7] Additionally, formation of R.R.Y. triplex conformations have been observed at the Mbr of the bcl-2 gene which is associated with follicular lymphomas.[7][8]The interaction between two triplex structures, termed “sticky” DNA interrupting transcription of the frataxin gene, has furthermore been suggested to be the basis for the instability resulting in Friedreich's ataxia.[9][10]

- Briefly touch on what breakpoints are

- “Found neighboring polypurine mirror-repeat h-dna forming sequences” is difficult to understand possibly restructure

- How is this neighboring significant?

- Triple helix is not referred as H-DNA until last paragraph and is not explained that H-DNA is referring to the triple helix

- May be helpful to link Z-DNA wiki page when mentioning it

- Follicular lymphomas - mention why the association of this could be significant (possibly medical research)

- Last sentence doesn't flow well, possibly move comma from after “gene” to after “DNA” ?

- Overall very concise and well-written!

Other comments-

Are you keeping this part from the original page? I think it is important and should be kept in (students added it last year).

Intramolecular triplex DNA is formed from a duplex with homopurine and homopyrimidine strands with mirror repeat symmetry.[1] The level of supercoiling in DNA influences the level of intramolecular triplex formation.[2] There are two different types of intramolecular triplex DNA: H-DNA and H*-DNA. Formation of H-DNA is stabilized under acidic conditions and in the presence of divalent cations such as Mg2+. In this conformation, the homopyrimidine strand in the duplex bends back to bind to the purine strand in a parallel fashion. The base triads used to stabilize this conformation are T-A-*T and C-G*C+. The cytosine in this base triad needs to be protonated in order to form this intramolecular triple helix, which is why this conformation is stabilized under acidic conditions.[3] H*-DNA has favorable formation conditions at neutral pH and in the presence of divalent cations.[4] This intramolecular conformation is formed from the binding of the homopurine and purine strand of the duplex in an antiparallel fashion. It is stabilized by T-A*A and C-G*G base triplets.[5][6] — Preceding unsigned comment added by DrMZF (talk • contribs) 00:01, 16 November 2018 (UTC)

The figures are great! In addition to the figure and restructuring the page, is your major contribution the part on genetic instability? — Preceding unsigned comment added by DrMZF (talk • contribs) 00:04, 16 November 2018 (UTC)

Ian was responsible for the figures and really did do a great job. There is a different sandbox we've all been working on at https://en.wikipedia.org/wiki/User:DubOOIan/sandbox3

My main contribution was Genetic Instability and help reorganizing the original layout. I did some edits yesterday I thought was in this sandbox but it must have been in the group sandbox I linked to above. Ill make sure they get into this sandbox this time. Seaveys (talk) 16:54, 16 November 2018 (UTC)