User talk:Tclayshulte/sandbox

Secondary (Angelika): - I suggest putting in a different header for "Evolution," or just separating it from "Ecology." Evolution may be intimately related to ecology but they are different, unless I totally misunderstood.

- I also changed stressers to stressors.

- Here's a reference you can use if you want to add something about the hemoglobin of actinorhizal plants: Bhattacharya, Sanghati, et al. "Characterization of Haemoglobin from Actinorhizal Plants – an in Silico Approach." Journal of Biosciences, vol. 38, no. 4, Nov. 2013, pp. 777-787. <---This really helped! I was curious as to why there were no vesicles observed in that group.

- Complete the first sentence in "Frankia Genome"

- I added a dash for "thick-walled"

- For the introduction, you can start by explaining what actinorhizal symbiosis is, where it can be observed, and when it was first described.

- In "Evolution," can you specify which are the four Frankia lineages?

- For "Classification," maybe you can include how Frankia was isolated in 1956.

- I just moved my comments from your sandbox to here.

Maisidro (talk) 23:46, 27 November 2017 (UTC)Angelika

Secondary - Angelika

- I suggest changing "though" to "although" in "Actinorhizal plants [...] all infect different plants"

- Maybe it's more appropriate if you move Root Nodules and Intracellular/Intercellular infection under the heading "Ecology", and then move Classification under the "Introduction."

- I fixed the typo "interceullarly" to "intercellularly"

- Can you expand on "Helper Bacteria"? I think this is very relevant to our class.

- I also corrected some grammar, you can review them if you'd like.

Maisidro (talk) 01:07, 28 November 2017 (UTC)

Secondary - Angelika

- I've been looking for more articles and here's one that I've found: "Molecular Phylogenies of Plants and Frankia Support Multiple Origins of Actinorhizal Symbioses" by S.C. Jeong et al. (Article ID: mpev.1999.0692). The authors' experiment included analyzing phylogenies of actinorhizal plants and Frankia by using 16S rDNA and rbcL gene sequences. Some of their results showed that Frankia and actinorhizal plants did not strictly coevolve and that actinorhizal symbiosis has multiple origins.

- If you'd like to add something about flavonoids and actinorhizal symbiosis: "Differential Effects of Rare Specific Flavonoids on Compatible and Incompatible Strains in the Myrica gale-Frankia Actinorhizal Symbiosis" by Jean Popovici et al. (doi: 10.1128/AEM.02667-09). 'This work provides evidence for the involvement of plant secondary metabolites in determining symbiotic specificity and expands our understanding of the mechanisms, leading to the establishment of actinorhizal symbioses' --from the abstract.

- This article is on the older side but it's about nodule-specific gene expression in an actinorhizal plant: "Differential gene expression in an actinorhizal symbiosis: evidence for a nodule-specific cysteine proteinase" by Goetting-Minesky and Mullin (PubMed ID: 7937912). The authors isolated a sequence representing the mRNA of a cysteine proteinase from an Alnus glutinosa (an actinorhizal plant) nodule cDNA library and determined that its expression is nodule-specific after comparing its expression in root and leaf tissues. The results also showed that AgNOD-CP1 (deduced amino acid sequence of the cDNA insert of pAgNg2O3-11) is probably expressed as an actinorhizal nodulin, whose suggested functions include being 'part of a defense response to Frankia infection, a component of tissue remodeling in root and nodule tissues, an integral component of the symbiosis-induced cell cycle in the nodule, and an agent of recycling and thus conservation of nitrogenous compounds invested in the symbiosis.'

Maisidro (talk) 12/16